caa a22 4500 605505403 CHVBK 20210128100621.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6888-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6888-3 Analysis of the intestinal microbial community structure of healthy and long-living elderly residents in Gaotian Village of Liuyang City [Elektronische Daten] [Xiaomin Yu, Xiaoli Wu, Liang Qiu, Dengyuan Wang, Min Gan, Xingxing Chen, Hua Wei, Feng Xu] Gaotian, one typical conservative village in rural area of South China, is differentiated from other adjacent village for its longevity and health situation of residents. To ascertain the difference of intestinal microbial community between Gaotian and other region, high-throughput sequencing and systematical bioinformation analyses was adopted to compare 21 samples in long life group with 28 in control group. The α diversity showed that the diversity of species of intestinal flora of Gaotian villagers was higher than that of control group, while the β diversity showed that the similarity of intestinal flora for Gaotian residents was also much higher than that of control group. OTU cluster analysis and Venn diagram showed that the intestinal microbial community of Gaotian villagers is different from that of control group. To quantitatively compare the main flora constitution in all samples, real-time PCR was performed, and the results showed that the biomass of Enterococcus, Lactobacillus, Enterobacteriaceae, Clostridium perfringens, and Bacteroides of Gaotian villages is generally significantly higher than that of control group. Remarkably, some special species, i.e., Methanobacterium, Butyricimonas, Deinococcus, and Streptococcaceae, have been found in Gaotian villagers. Overall, this study lays a preparatory basis for exploration of the resources of special species from healthy and long-living elderly Gaotian villagers and for proposal of a hypothesis, namely, the diversity in intestinal flora of Gaotian might contribute to the longevity and health of local residents. Further study should be focused on screening and functional evaluation of the special species in the long-life residents. Springer-Verlag Berlin Heidelberg, 2015 High throughput nationallicence Longevity nationallicence Flora nationallicence Diversity nationallicence Yu Xiaomin State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut Wu Xiaoli Jiangxi University of Traditional Chinese Medicine, 330004, Nanchang, China aut Qiu Liang State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut Wang Dengyuan State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut Gan Min State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut Chen Xingxing State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut Wei Hua State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut Xu Feng Jiangxi-OAI Joint Research Institute, Nanchang University, 330047, Nanchang, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9085-9095 0175-7598 99:21<9085 2015 99 253 https://doi.org/10.1007/s00253-015-6888-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6888-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Xiaomin State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xiaoli Jiangxi University of Traditional Chinese Medicine, 330004, Nanchang, China aut NATIONALLICENCE 700 1- Qiu Liang State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Dengyuan State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Gan Min State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xingxing State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Wei Hua State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Xu Feng Jiangxi-OAI Joint Research Institute, Nanchang University, 330047, Nanchang, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9085-9095 0175-7598 99:21<9085 2015 99 253