caa a22 4500 605505438 CHVBK 20210128100622.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6825-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6825-5 Efficient two-step chemo-enzymatic synthesis of all- trans -retinyl palmitate with high substrate concentration and product yield [Elektronische Daten] [Zhi-Qiang Liu, Ling-Mei Zhou, Peng Liu, Peter Baker, Shan-Shan Liu, Ya-Ping Xue, Ming Xu, Yu-Guo Zheng] A new two-step chemo-enzymatic approach for highly efficient synthesis of all-trans-retinyl palmitate is constructed in this study. In the first step, retinyl acetate as starting material was fully hydrolyzed to retinol by potassium hydroxide. In the hydrolysis system, anhydrous ethanol was the best co-solvent to increase the solubility of retinyl acetate. The addition amounts of 5M potassium hydroxide and anhydrous ethanol were 8 and 10mL against 10g retinyl acetate, respectively, and 100% hydrolysis rate was obtained. In the second step, esterification was catalyzed by immobilized lipase on macroporous acrylic resin AB-8 using the extracted retinol and palmitic acid as substrates in non-aqueous system. After optimization, the parameters of esterification reaction were confirmed as follows: non-aqueous solvent was selected as n-hexane, washing times of extraction solution was four times, retinol concentration was 300 g/L, substrate molar ratio of retinol to palmitic acid was 1:1.1, the amount of immobilized enzyme was 10g/L, and the esterification temperature was 30°C. Under the optimal conditions, this protocol resulted in a 97.5% yield of all-trans-retinyl palmitate in 700-L reactor. After purification, all-trans-retinyl palmitate was obtained with above 99% of purity and 88% of total recovery rate. This methodology provides a promising strategy for the large-scale production of all-trans-retinyl palmitate. Springer-Verlag Berlin Heidelberg, 2015 Lipase nationallicence Immobilization nationallicence Esterification nationallicence Chemo-enzymatic synthesis nationallicence All- trans -retinyl palmitate nationallicence Liu Zhi-Qiang Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Zhou Ling-Mei Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Liu Peng Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Baker Peter Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Liu Shan-Shan Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Xue Ya-Ping Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Xu Ming Zhejiang Laiyi Biotechnology Co. Ltd., 312400, Shengzhou, Zhejiang, People's Republic of China aut Zheng Yu-Guo Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 8891-8902 0175-7598 99:21<8891 2015 99 253 https://doi.org/10.1007/s00253-015-6825-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6825-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Zhi-Qiang Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Ling-Mei Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Peng Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Baker Peter Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shan-Shan Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xue Ya-Ping Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xu Ming Zhejiang Laiyi Biotechnology Co. Ltd., 312400, Shengzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Yu-Guo Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 8891-8902 0175-7598 99:21<8891 2015 99 253