caa a22 4500 605505446 CHVBK 20210128100622.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6798-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6798-4 Decolorization characteristics of a newly isolated salt-tolerant Bacillus sp. strain and its application for azo dye-containing wastewater in immobilized form [Elektronische Daten] [Lei Yu, Xiao-yu Zhang, Qing-wen Tang, Jia Li, Tian Xie, Chang Liu, Ming-yue Cao, Rui-chang Zhang, Shi Wang, Jin-mei Hu, Wei-chuan Qiao, Wen-wei Li, Hong-hua Ruan] Strain CICC 23870 capable of decolorization of various azo dyes under high saline conditions was isolated from saline-alkali soil. The oxygen-insensitive azoreductase in crude extracts exhibited a wide substrate adaptively in the presence of NADH as a cofactor. The decolorization process by free cells followed first-order kinetics, with a high Methyl Orange (MO) tolerance concentration up to 100mgl−1 estimated by Haldane model. The average decolorization rate of free cell system was 26.30mgg−1h−1 at initial MO concentration of 32.7mgl−1. However, the values for the systems of immobilized cells (4mm) in alginate, alginate and nano-TiO2, and alginate and powered activated carbon (PAC) were 6.83, 4.64, and 11.34mgg−1h−1, respectively. The effective diffusion factors in the tree different matrices were calculated by diffusion-based mathematic model. The diffusion step controls the overall decolorization rate, and the effective diffusion coefficients varied with internal structure of the bead matrices. The diffusion coefficients were increased from 4.98 × 10−9 to 2.25 × 10−8cm2s−1 when PAC was added, but decreased to 6.62 × 10−10cm2s−1 when nano-TiO2 was added. The immobilized matrices could be reused for at least three cycles but with a decreased decolorization rate, possibly due to the breakage of beads at the end of each cycle, which led to the loss of immobilized bacteria. Springer-Verlag Berlin Heidelberg, 2015 Anaerobic decolorization nationallicence Salt-tolerant bacteria nationallicence Immobilization nationallicence Diffusion modeling nationallicence Yu Lei College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Zhang Xiao-yu College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Tang Qing-wen College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Li Jia College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Xie Tian College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Liu Chang College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Cao Ming-yue College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Zhang Rui-chang Key Laboratory of soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Wang Shi College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Hu Jin-mei College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Qiao Wei-chuan College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Li Wen-wei CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut Ruan Hong-hua College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9277-9287 0175-7598 99:21<9277 2015 99 253 https://doi.org/10.1007/s00253-015-6798-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6798-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Lei College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Xiao-yu College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Tang Qing-wen College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Li Jia College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Xie Tian College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Liu Chang College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Cao Ming-yue College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Rui-chang Key Laboratory of soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Wang Shi College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Hu Jin-mei College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Qiao Wei-chuan College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 700 1- Li Wen-wei CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut NATIONALLICENCE 700 1- Ruan Hong-hua College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9277-9287 0175-7598 99:21<9277 2015 99 253