caa a22 4500 605505527 CHVBK 20210128100622.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6786-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6786-8 Differential modulation of avian β-defensin and Toll-like receptor expression in chickens infected with infectious bronchitis virus [Elektronische Daten] [Yang Xu, Tingting Zhang, Qianqian Xu, Zongxi Han, Shuling Liang, Yuhao Shao, Deying Ma, Shengwang Liu] The host innate immune response either clears invading viruses or allows the adaptive immune system to establish an effective antiviral response. In this study, both pathogenic (passage 3, P3) and attenuated (P110) infectious bronchitis virus (IBV) strains were used to study the immune responses of chicken to IBV infection. Expression of avian β-defensins (AvBDs) and Toll-like receptors (TLRs) in 16 tissues of chicken were compared at 7days PI. The results showed that P3 infection upregulated the expression of AvBDs, including AvBD2, 4, 5, 6, 9, and 12, while P110 infection downregulated the expression of AvBDs, including AvBD3, 4, 5, 6, and 9 in most tissues. Meanwhile, the expression level of several TLRs showed a general trend of upregulation in the tissues of P3-infected chickens, while they were downregulated in the tissues of P110-infected chickens. The result suggested that compared with the P110 strain, the P3 strain induced a more pronounced host innate immune response. Furthermore, we observed that recombinant AvBDs (including 2, 6, and 12) demonstrated obvious anti-viral activity against IBV in vitro. Our findings contribute to the proposal that IBV infection induces an increase in the messenger RNA (mRNA) expression of some AvBDs and TLRs, which suggests that AvBDs may play significant roles in the resistance of chickens to IBV replication. Springer-Verlag Berlin Heidelberg, 2015 Chicken nationallicence Avian β-defensins nationallicence Toll-like receptors nationallicence Infectious bronchitis virus nationallicence Xu Yang College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Zhang Tingting College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Xu Qianqian College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Han Zongxi Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut Liang Shuling College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Shao Yuhao Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut Ma Deying College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Liu Shengwang Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9011-9024 0175-7598 99:21<9011 2015 99 253 https://doi.org/10.1007/s00253-015-6786-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6786-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Yang College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Tingting College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Xu Qianqian College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Han Zongxi Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Liang Shuling College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Shao Yuhao Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Ma Deying College of Animal Science and Technology, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shengwang Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9011-9024 0175-7598 99:21<9011 2015 99 253