caa a22 4500 605505586 CHVBK 20210128100622.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6769-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6769-9 Comparative phenomics and targeted use of genomics reveals variation in carbon and nitrogen assimilation among different Brettanomyces bruxellensis strains [Elektronische Daten] [S. Crauwels, A. Van Assche, R. de Jonge, A. Borneman, C. Verreth, P. Troels, G. De Samblanx, K. Marchal, Y. Van de Peer, K. Willems, K. Verstrepen, C. Curtin, B. Lievens] Recent studies have suggested a correlation between genotype groups of Brettanomyces bruxellensis and their source of isolation. To further explore this relationship, the objective of this study was to assess metabolic differences in carbon and nitrogen assimilation between different B. bruxellensis strains from three beverages, including beer, wine, and soft drink, using Biolog Phenotype Microarrays. While some similarities of physiology were noted, many traits were variable among strains. Interestingly, some phenotypes were found that could be linked to strain origin, especially for the assimilation of particular α- and β-glycosides as well as α- and β-substituted monosaccharides. Based upon gene presence or absence, an α-glucosidase and β-glucosidase were found explaining the observed phenotypes. Further, using a PCR screen on a large number of isolates, we have been able to specifically link a genomic deletion to the beer strains, suggesting that this region may have a fitness cost for B. bruxellensis in certain fermentation systems such as brewing. More specifically, none of the beer strains were found to contain a β-glucosidase, which may have direct impacts on the ability for these strains to compete with other microbes or on flavor production. Springer-Verlag Berlin Heidelberg, 2015 α-Glucosidase nationallicence β-Glucosidase nationallicence Biolog nationallicence Dekkera bruxellensis nationallicence Niche adaptation nationallicence Phenotypic and genetic diversity nationallicence Crauwels S. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut Van Assche A. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut de Jonge R. Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052, Ghent, Belgium aut Borneman A. The Australian Wine Research Institute, Glen Osmond, PO Box 197, 5064, Adelaide, SA, Australia aut Verreth C. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut Troels P. White Labs, 9495 Candida Street, 92126, San Diego, CA, USA aut De Samblanx G. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut Marchal K. Department of Plant Biotechnology and Bioinformatics and Department of Information Technology, IMinds, Ghent University, Technologiepark 927, B-9052, Ghent, Belgium aut Van de Peer Y. Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052, Ghent, Belgium aut Willems K. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut Verstrepen K. VIB Lab for Systems Biology and Centre of Microbial and Plant Genetics (CMPG) Lab for Genetics and Genomics, KU Leuven, M2S, Gaston Geenslaan 1, B-3001, Leuven, Belgium aut Curtin C. The Australian Wine Research Institute, Glen Osmond, PO Box 197, 5064, Adelaide, SA, Australia aut Lievens B. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9123-9134 0175-7598 99:21<9123 2015 99 253 https://doi.org/10.1007/s00253-015-6769-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6769-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Crauwels S. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut NATIONALLICENCE 700 1- Van Assche A. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut NATIONALLICENCE 700 1- de Jonge R. Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052, Ghent, Belgium aut NATIONALLICENCE 700 1- Borneman A. The Australian Wine Research Institute, Glen Osmond, PO Box 197, 5064, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Verreth C. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut NATIONALLICENCE 700 1- Troels P. White Labs, 9495 Candida Street, 92126, San Diego, CA, USA aut NATIONALLICENCE 700 1- De Samblanx G. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut NATIONALLICENCE 700 1- Marchal K. Department of Plant Biotechnology and Bioinformatics and Department of Information Technology, IMinds, Ghent University, Technologiepark 927, B-9052, Ghent, Belgium aut NATIONALLICENCE 700 1- Van de Peer Y. Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052, Ghent, Belgium aut NATIONALLICENCE 700 1- Willems K. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut NATIONALLICENCE 700 1- Verstrepen K. VIB Lab for Systems Biology and Centre of Microbial and Plant Genetics (CMPG) Lab for Genetics and Genomics, KU Leuven, M2S, Gaston Geenslaan 1, B-3001, Leuven, Belgium aut NATIONALLICENCE 700 1- Curtin C. The Australian Wine Research Institute, Glen Osmond, PO Box 197, 5064, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Lievens B. Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, M2S, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne Waver, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9123-9134 0175-7598 99:21<9123 2015 99 253