caa a22 4500 605505594 CHVBK 20210128100622.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6827-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6827-3 Dominance of rumen microorganisms during cheese whey acidification: acidogenesis can be governed by a rare Selenomonas lacticifex -type fermentation [Elektronische Daten] [Spyridon Ntougias, George Tsiamis, Despoina Soultani, Paraschos Melidis] The microbial basis of acidification process during spontaneous cheese whey wastewater fermentation was decrypted by implementing both culture-dependent and culture-independent techniques. Lac tobacillus and Bifidobacterium were the predominant taxa among the microbiota growing on MRS (deMan, Rogosa, and Sharpe), while Kazachstania unispora and Dekkera anomala yeast species were also isolated. Almost all Lactobacillus isolates were heterofermentative that could ferment glucose and lactose, with most of them being related to Lactobacillus hilgardii (99.0-100% similarity). By employing fluorescence techniques, the dominance of long crescent-shaped bacteria in the acidogenic sludge was observed. Temperature gradient gel electrophoresis (TGGE), clone library, and next-generation sequencing techniques revealed the dominance of Selenomonas lacticifex. Based on Illumina data, Selenomonas in the continuous stirred-tank reactor (CSTR) represented 70.13±4.64% of the bacterial reads, while other Veillonellaceae taxa (Megasphaera and Pectinatus) represented a notable proportion (6.54%). Prevotella was only detected by Illumina sequencing as an important constituent of the microbial population (14.97±1.71%). Budding yeasts represented 97% of the fungal population in the CSTR, with Yarrowia strains representing 88.85±5.52% of the fungal reads. Spontaneous cheese whey acidification can favor the dominance of rumen bacteria and here was driven by the rarely reported S. lacticifex-type fermentation, which should be taken into consideration during evaluation of acidogenesis in process simulation and modelling. Moreover, the important nervonic acid content detected indicates that acidogenic sludge can be used as a source for the production of high value-added biomedical substrates. Springer-Verlag Berlin Heidelberg, 2015 Fermentative microorganisms nationallicence Selenomonas lacticifex nationallicence Acidogenesis nationallicence Rumen bacteria nationallicence Veillonellaceae nationallicence Cheese whey wastewater nationallicence Ntougias Spyridon Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece aut Tsiamis George Department of Environmental and Natural Resources Management, University of Patras, 2 Seferi str., 30100, Agrinio, Greece aut Soultani Despoina Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece aut Melidis Paraschos Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9309-9318 0175-7598 99:21<9309 2015 99 253 https://doi.org/10.1007/s00253-015-6827-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6827-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ntougias Spyridon Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece aut NATIONALLICENCE 700 1- Tsiamis George Department of Environmental and Natural Resources Management, University of Patras, 2 Seferi str., 30100, Agrinio, Greece aut NATIONALLICENCE 700 1- Soultani Despoina Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece aut NATIONALLICENCE 700 1- Melidis Paraschos Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9309-9318 0175-7598 99:21<9309 2015 99 253