caa a22 4500 605505616 CHVBK 20210128100622.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6761-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6761-4 Engineered Deinococcus radiodurans R1 with NiCoT genes for bioremoval of trace cobalt from spent decontamination solutions of nuclear power reactors [Elektronische Daten] [Raghu Gogada, Surya Singh, Shanti Lunavat, Maruthi Pamarthi, Agnes Rodrigue, Balaji Vadivelu, Prakash-Babu Phanithi, Venkateswaran Gopala, Shree Apte] The aim of the present work was to engineer bacteria for the removal of Co in contaminated effluents. Radioactive cobalt (60Co) is known as a major contributor for person-sievert budgetary because of its long half-life and high γ-energy values. Some bacterial Ni/Co transporter (NiCoT) genes were described to have preferential uptake for cobalt. In this study, the NiCoT genes nxiA and nvoA from Rhodopseudomonas palustris CGA009 (RP) and Novosphingobium aromaticivorans F-199 (NA), respectively, were cloned under the control of the groESL promoter. These genes were expressed in Deinococcus radiodurans in reason of its high resistance to radiation as compared to other bacterial strains. Using qualitative real time-PCR, we showed that the expression of NiCoT-RP and NiCoT-NA is induced by cobalt and nickel. The functional expression of these genes in bioengineered D. radiodurans R1 strains resulted in >60% removal of 60Co (≥5.1 nM) within 90min from simulated spent decontamination solution containing 8.5 nM of Co, even in the presence of >10mM of Fe, Cr, and Ni. D. radiodurans R1 (DR-RP and DR-NA) showed superior survival to recombinant E. coli (ARY023) expressing NiCoT-RP and NA and efficiency in Co remediation up to 6.4kGy. Thus, the present study reports a remarkable reduction in biomass requirements (2kg) compared to previous studies using wild-type bacteria (50kg) or ion-exchanger resins (8000kg) for treatment of ~105-l spent decontamination solutions (SDS). Springer-Verlag Berlin Heidelberg, 2015 Bioengineering nationallicence Bioremediation nationallicence NiCoT genes nationallicence Trace cobalt removal nationallicence Spent decontamination solutions (SDS) nationallicence Nuclear power reactors nationallicence Gogada Raghu Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, 500046, Hyderabad, India aut Singh Surya Department of Biochemistry, Osmania University, 500 007, Hyderabad, India aut Lunavat Shanti Department of Biochemistry, Osmania University, 500 007, Hyderabad, India aut Pamarthi Maruthi Department of Biochemistry, Osmania University, 500 007, Hyderabad, India aut Rodrigue Agnes Université Lyon 1, INSA de Lyon, CNRS, UMR5240, Microbiologie, Adaptation et Pathogénie, Université de Lyon, 69621, Villeurbanne, France aut Vadivelu Balaji Water Steam Chemistry Division, Bhabha Atomic Research Center, 400 085, Mumbai, India aut Phanithi Prakash-Babu Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, 500046, Hyderabad, India aut Gopala Venkateswaran Analytical Chemistry Division, Bhabha Atomic Research Center, 400 085, Mumbai, India aut Apte Shree Molecular Biology Division, Bhabha Atomic Research Center, 400 085, Mumbai, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9203-9213 0175-7598 99:21<9203 2015 99 253 https://doi.org/10.1007/s00253-015-6761-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6761-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gogada Raghu Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, 500046, Hyderabad, India aut NATIONALLICENCE 700 1- Singh Surya Department of Biochemistry, Osmania University, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Lunavat Shanti Department of Biochemistry, Osmania University, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Pamarthi Maruthi Department of Biochemistry, Osmania University, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Rodrigue Agnes Université Lyon 1, INSA de Lyon, CNRS, UMR5240, Microbiologie, Adaptation et Pathogénie, Université de Lyon, 69621, Villeurbanne, France aut NATIONALLICENCE 700 1- Vadivelu Balaji Water Steam Chemistry Division, Bhabha Atomic Research Center, 400 085, Mumbai, India aut NATIONALLICENCE 700 1- Phanithi Prakash-Babu Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, 500046, Hyderabad, India aut NATIONALLICENCE 700 1- Gopala Venkateswaran Analytical Chemistry Division, Bhabha Atomic Research Center, 400 085, Mumbai, India aut NATIONALLICENCE 700 1- Apte Shree Molecular Biology Division, Bhabha Atomic Research Center, 400 085, Mumbai, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9203-9213 0175-7598 99:21<9203 2015 99 253