caa a22 4500 605505632 CHVBK 20210128100623.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6907-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6907-4 Effects of constant or dynamic low anode potentials on microbial community development in bioelectrochemical systems [Elektronische Daten] [Hengjing Yan, Matthew Yates, John Regan] In bioelectrochemical systems, exoelectrogenic bacteria respire with anode electrodes as their extracellular electron acceptor; therefore, lower anode potentials can reduce the energy gain to each microbe and select against ones that are not able to respire at a lower potential range. Often fully developed anode communities are compared across bioelectrochemical systems with set anode potentials or fixed external resistances as different operational conditions. However, the comparative effect of the resulting constantly low versus dynamically low anode potentials on the development of anode microbial communities as well as the final cathode microbial communities has not been directly demonstrated. In this study, we used a low fixed anode potential of −250mV and a higher-current control potential of −119mV vs. Standard Hydrogen Electrode to approximately correspond with the negative peak anode potential values obtained from microbial fuel cells operated with fixed external resistances of 1kΩ and 47Ω, respectively. Pyrosequencing data from a 2-month time series show that a lower set anode potential resulted in a more diverse community than the higher- and variable-potential systems, likely due to the hindered enrichment of a Geobacter-dominated community with limited energy gain at this set potential. In this case, it appears that the selective pressure caused by the low set potential was counteracted by the low energy gain over a 2-month time scale. The air cathode microbial community with constant low anode potentials showed delayed enrichment of denitrifiers or perchlorate-reducing bacteria compared to the fixed external resistance condition. Springer-Verlag Berlin Heidelberg, 2015 Geobacter nationallicence Microbial community nationallicence Potentiostatic control nationallicence Anode potentials nationallicence Pyrosequencing nationallicence Microbial fuel cells nationallicence Yan Hengjing Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett, 16802, University Park, PA, USA aut Yates Matthew Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett, 16802, University Park, PA, USA aut Regan John Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett, 16802, University Park, PA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9319-9329 0175-7598 99:21<9319 2015 99 253 https://doi.org/10.1007/s00253-015-6907-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6907-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Hengjing Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Yates Matthew Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Regan John Department of Civil and Environmental Engineering, The Pennsylvania State University, 212 Sackett, 16802, University Park, PA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 9319-9329 0175-7598 99:21<9319 2015 99 253