Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605505683 | ||
| 003 | CHVBK | ||
| 005 | 20210128100623.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6619-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6619-9 | ||
| 245 | 0 | 0 | |a Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose |h [Elektronische Daten] |c [Fang Yang, Xiaofeng Yang, Zhe Li, Chenyu Du, Jufang Wang, Shuang Li] |
| 520 | 3 | |a Thermoanaerobacterium aotearoense P8G3#4 produced β-glucosidase (BGL) intracellularly when grown in liquid culture on cellobiose. The gene bgl, encoding β-glucosidase, was cloned and sequenced. Analysis revealed that the bgl contained an open reading frame of 1314bp encoding a protein of 446 amino acid residues, and the product belonged to the glycoside hydrolase family 1 with the canonical glycoside hydrolase family 1 (GH1) (β/α)8 TIM barrel fold. Expression of pET-bgl together with a chaperone gene cloned in vector pGro7 in Escherichia coli dramatically enhanced the crude enzyme activity to a specific activity of 256.3U/mg wet cells, which resulted in a 9.2-fold increase of that obtained from the expression without any chaperones. The purified BGL exhibited relatively high thermostability and pH stability with its highest activity at 60°C and pH 6.0. In addition, the activities of BGL were remarkably stimulated by the addition of 5mM Na+ or K+. The enzyme showed strong ability to hydrolyze cellobiose with a K m and V max of 25.45mM and 740.5U/mg, respectively. The BGL was activated by glucose at concentration varying from 50 to 250mM and tolerant to glucose inhibition with a K i of 800mM glucose. The supplement of the purified BGL to the sugarcane bagasse hydrolysis mixture containing a commercial cellulase resulted in about 20% enhancement of the released reducing sugars. These properties of the purified BGL should have important practical implication in its potential applications for better industrial production of glucose or bioethanol started from lignocellulosic biomass. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a β-Glucosidase |2 nationallicence | |
| 690 | 7 | |a Thermoanaerobacterium aotearoense P8G3#4 |2 nationallicence | |
| 690 | 7 | |a Glucose tolerance |2 nationallicence | |
| 690 | 7 | |a Cellobiose degradation |2 nationallicence | |
| 690 | 7 | |a Chaperones |2 nationallicence | |
| 700 | 1 | |a Yang |D Fang |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | |
| 700 | 1 | |a Yang |D Xiaofeng |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Zhe |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | |
| 700 | 1 | |a Du |D Chenyu |u School of Applied Sciences, The University of Huddersfield, Queensgate, HD1 3DH, Huddersfield, UK |4 aut | |
| 700 | 1 | |a Wang |D Jufang |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Shuang |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 8903-8915 |x 0175-7598 |q 99:21<8903 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6619-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6619-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Fang |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Xiaofeng |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Zhe |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Du |D Chenyu |u School of Applied Sciences, The University of Huddersfield, Queensgate, HD1 3DH, Huddersfield, UK |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Jufang |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Shuang |u Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 8903-8915 |x 0175-7598 |q 99:21<8903 |1 2015 |2 99 |o 253 | ||