Fine-tuning recA expression in Staphylococcus aureus for antimicrobial photoinactivation: importance of photo-induced DNA damage in the photoinactivation mechanism
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 60550573X | ||
| 003 | CHVBK | ||
| 005 | 20210128100623.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6863-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6863-z | ||
| 245 | 0 | 0 | |a Fine-tuning recA expression in Staphylococcus aureus for antimicrobial photoinactivation: importance of photo-induced DNA damage in the photoinactivation mechanism |h [Elektronische Daten] |c [Mariusz Grinholc, Aleksandra Rodziewicz, Katarzyna Forys, Aleksandra Rapacka-Zdonczyk, Anna Kawiak, Anna Domachowska, Grzegorz Golunski, Christiane Wolz, Lili Mesak, Karsten Becker, Krzysztof Bielawski] |
| 520 | 3 | |a Bacterial cell envelope is generally accepted as the primary target for a photo-induced oxidative stress. It is plausible that DNA damage occurs during the antimicrobial photoinactivation. Here we investigate the correlation between DNA damage and photoinactivation by evaluating the level of RecA-based DNA repair system in Staphylococcus aureus. By using exogenous photosensitizers (new methylene blue (NMB), toluidine blue O (TBO), 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) (TMPyP), zinc phthalocyanine (ZnPc), Rose Bengal (RB)) and ALA-induced endogenous porphyrin-dependent blue light (405nm), several outcomes were observed: (i) an increase of DNA damage (from gel electrophoresis in DNA damage assay), (ii) an increase of recA expression (luminescence assay in recA-lux strain), and (iii) an increase of RecA protein level (Western blotting). When recA expression was repressed by novobiocin, or abolished by deleting the gene, S. aureus susceptibility towards photoinactivation was increased at approximately a hundred-fold. The absence of RecA increases DNA damage to yield bactericidal effect. In novobiocin-resistant mutant (gyrB), as opposed to wild type, neither RecA protein level nor cell's susceptibility was affected by photoinactivation (when novobiocin is present). This is to suggest that GyrB-dependent inhibition mediated recA repression. Therefore, we have established the role of RecA in DNA damage during photoinactivation. With the use of rifampicin mutation frequency and Ames tests, we demonstrated that photoinactivation did not increase S. aureus mutagenesis and potentially is not mutagenic toward eukaryotic cells. The results suggest that the treatment is considered safe. In conclusion, we provide an evidence that recA inhibitor may serve as therapeutic adjuvant for antimicrobial photoinactivation. Clinical relevance of our findings warrants further investigations. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a DNA damage |2 nationallicence | |
| 690 | 7 | |a Endogenous porphyrins |2 nationallicence | |
| 690 | 7 | |a Mutagenesis |2 nationallicence | |
| 690 | 7 | |a Photoinactivation |2 nationallicence | |
| 690 | 7 | |a RecA |2 nationallicence | |
| 690 | 7 | |a Staphylococcus aureus |2 nationallicence | |
| 700 | 1 | |a Grinholc |D Mariusz |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Rodziewicz |D Aleksandra |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Forys |D Katarzyna |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Rapacka-Zdonczyk |D Aleksandra |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Kawiak |D Anna |u Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Domachowska |D Anna |u Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Golunski |D Grzegorz |u Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 700 | 1 | |a Wolz |D Christiane |u Interfaculty Institute of Microbiology and Infection Medicine, University of Tubingen, Wlfriede-Aulhorn-Strasse 6, 72076, Tubingen, Germany |4 aut | |
| 700 | 1 | |a Mesak |D Lili |u Outreach, Research Training and Minority Science Program, Ayala School of Biological Sciences, University of California, 333 Steinhaus Hall, 92697-2525, Irvine, CA, USA |4 aut | |
| 700 | 1 | |a Becker |D Karsten |u Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149, Münster, Germany |4 aut | |
| 700 | 1 | |a Bielawski |D Krzysztof |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 9161-9176 |x 0175-7598 |q 99:21<9161 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6863-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6863-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grinholc |D Mariusz |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rodziewicz |D Aleksandra |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Forys |D Katarzyna |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rapacka-Zdonczyk |D Aleksandra |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawiak |D Anna |u Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Domachowska |D Anna |u Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Golunski |D Grzegorz |u Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wolz |D Christiane |u Interfaculty Institute of Microbiology and Infection Medicine, University of Tubingen, Wlfriede-Aulhorn-Strasse 6, 72076, Tubingen, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mesak |D Lili |u Outreach, Research Training and Minority Science Program, Ayala School of Biological Sciences, University of California, 333 Steinhaus Hall, 92697-2525, Irvine, CA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Becker |D Karsten |u Institute of Medical Microbiology, University Hospital Münster, Domagkstr. 10, 48149, Münster, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bielawski |D Krzysztof |u Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 9161-9176 |x 0175-7598 |q 99:21<9161 |1 2015 |2 99 |o 253 | ||