Screening, identification, and characterization of a GH43 family β-xylosidase/α-arabinofuranosidase from a compost microbial metagenome
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| 008 | 210128e20151101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6647-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6647-5 | ||
| 245 | 0 | 0 | |a Screening, identification, and characterization of a GH43 family β-xylosidase/α-arabinofuranosidase from a compost microbial metagenome |h [Elektronische Daten] |c [Tomohiko Matsuzawa, Satoshi Kaneko, Katsuro Yaoi] |
| 520 | 3 | |a A putative glycoside hydrolase family 43 β-xylosidase/α-arabinofuranosidase (CoXyl43) that promotes plant biomass saccharification was isolated via functional screening of a compost microbial metagenomic library and characterized. CoXyl43 promoted the saccharification of plant biomasses, including xylans (xylan and arabinoxylan), rice straw, and Erianthus, by degrading xylooligosaccharide residues to monosaccharide residues. The recombinant CoXyl43 protein exhibited both β-xylosidase and α-arabinofuranosidase activities for chromogenic substrates, with optimal activity at pH 7.5 and 55°C. Both of these activities were inactivated by ethanol, dimethylsulfoxide, and zinc and copper ions but were activated by manganese ions. Only the β-xylosidase activity of recombinant CoXyl43 was enhanced in the presence of calcium ions. These results indicate that CoXyl43 exhibits unique enzymatic properties useful for biomass saccharification. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a β-xylosidase |2 nationallicence | |
| 690 | 7 | |a α-arabinofuranosidase |2 nationallicence | |
| 690 | 7 | |a Biomass |2 nationallicence | |
| 690 | 7 | |a Metagenome |2 nationallicence | |
| 690 | 7 | |a Glycoside hydrolase family 43 |2 nationallicence | |
| 700 | 1 | |a Matsuzawa |D Tomohiko |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan |4 aut | |
| 700 | 1 | |a Kaneko |D Satoshi |u Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, 305-8642, Tsukuba, Ibaraki, Japan |4 aut | |
| 700 | 1 | |a Yaoi |D Katsuro |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 8943-8954 |x 0175-7598 |q 99:21<8943 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6647-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6647-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Matsuzawa |D Tomohiko |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kaneko |D Satoshi |u Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, 305-8642, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yaoi |D Katsuro |u Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 8943-8954 |x 0175-7598 |q 99:21<8943 |1 2015 |2 99 |o 253 | ||