caa a22 4500 605505772 CHVBK 20210128100623.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6647-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6647-5 Screening, identification, and characterization of a GH43 family β-xylosidase/α-arabinofuranosidase from a compost microbial metagenome [Elektronische Daten] [Tomohiko Matsuzawa, Satoshi Kaneko, Katsuro Yaoi] A putative glycoside hydrolase family 43 β-xylosidase/α-arabinofuranosidase (CoXyl43) that promotes plant biomass saccharification was isolated via functional screening of a compost microbial metagenomic library and characterized. CoXyl43 promoted the saccharification of plant biomasses, including xylans (xylan and arabinoxylan), rice straw, and Erianthus, by degrading xylooligosaccharide residues to monosaccharide residues. The recombinant CoXyl43 protein exhibited both β-xylosidase and α-arabinofuranosidase activities for chromogenic substrates, with optimal activity at pH 7.5 and 55°C. Both of these activities were inactivated by ethanol, dimethylsulfoxide, and zinc and copper ions but were activated by manganese ions. Only the β-xylosidase activity of recombinant CoXyl43 was enhanced in the presence of calcium ions. These results indicate that CoXyl43 exhibits unique enzymatic properties useful for biomass saccharification. Springer-Verlag Berlin Heidelberg, 2015 β-xylosidase nationallicence α-arabinofuranosidase nationallicence Biomass nationallicence Metagenome nationallicence Glycoside hydrolase family 43 nationallicence Matsuzawa Tomohiko Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut Kaneko Satoshi Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, 305-8642, Tsukuba, Ibaraki, Japan aut Yaoi Katsuro Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 8943-8954 0175-7598 99:21<8943 2015 99 253 https://doi.org/10.1007/s00253-015-6647-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6647-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsuzawa Tomohiko Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kaneko Satoshi Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, 305-8642, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Yaoi Katsuro Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, 305-8566, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/21(2015-11-01), 8943-8954 0175-7598 99:21<8943 2015 99 253