Improved ethanol production from biomass by a rumen metagenomic DNA fragment expressed in Escherichia coli MS04 during fermentation
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6801-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6801-0 | ||
| 245 | 0 | 0 | |a Improved ethanol production from biomass by a rumen metagenomic DNA fragment expressed in Escherichia coli MS04 during fermentation |h [Elektronische Daten] |c [Inés Loaces, Vanesa Amarelle, Iván Muñoz-Gutierrez, Elena Fabiano, Alfredo Martinez, Francisco Noya] |
| 520 | 3 | |a With the aim of improving current ethanologenic Escherichia coli strains, we screened a metagenomic library from bovine ruminal fluid for cellulolytic enzymes. We isolated one fosmid, termed Csd4, which was able to confer to E. coli the ability to grow on complex cellulosic material as the sole carbon source such as avicel, carboxymethyl cellulose, filter paper, pretreated sugarcane bagasse, and xylan. Glucanolytic activity obtained from E. coli transformed with Csd4 was maximal at 24h of incubation and was inhibited when glucose or xylose were present in the media. The 34,406-bp DNA fragment of Csd4 was completely sequenced, and a putative endoglucanase, a xylosidase/arabinosidase, and a laccase gene were identified. Comparison analysis revealed that Csd4 derived from an organism closely related to Prevotella ruminicola, but no homologies were found with any of the genomes already sequenced. Csd4 was introduced into the ethanologenic E. coli MS04 strain and ethanol production from CMC, avicel, sugarcane bagasse, or filter paper was observed. Exogenously expressed β-glucosidase had a positie effect on cell growth in agreement with the fact that no putative β-glucosidase was found in Csd4. Ethanol production from sugarcane bagasse was improved threefold by Csd4 after saccharification by commercial Trichoderma reesei cellulases underlining the ability of Csd4 to act as a saccharification enhancer to reduce the enzymatic load and time required for cellulose deconstruction. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Rumen metagenomics |2 nationallicence | |
| 690 | 7 | |a CBP |2 nationallicence | |
| 690 | 7 | |a Cellulolytic enzymes |2 nationallicence | |
| 690 | 7 | |a Bioethanol |2 nationallicence | |
| 690 | 7 | |a Biomass hydrolysis |2 nationallicence | |
| 700 | 1 | |a Loaces |D Inés |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | |
| 700 | 1 | |a Amarelle |D Vanesa |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | |
| 700 | 1 | |a Muñoz-Gutierrez |D Iván |u Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P: 510-3., 62270, Cuernavaca, Morelos, México |4 aut | |
| 700 | 1 | |a Fabiano |D Elena |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | |
| 700 | 1 | |a Martinez |D Alfredo |u Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P: 510-3., 62270, Cuernavaca, Morelos, México |4 aut | |
| 700 | 1 | |a Noya |D Francisco |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 9049-9060 |x 0175-7598 |q 99:21<9049 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6801-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6801-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Loaces |D Inés |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Amarelle |D Vanesa |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Muñoz-Gutierrez |D Iván |u Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P: 510-3., 62270, Cuernavaca, Morelos, México |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fabiano |D Elena |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Martinez |D Alfredo |u Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P: 510-3., 62270, Cuernavaca, Morelos, México |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Noya |D Francisco |u Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, 11600, Montevideo, Uruguay |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/21(2015-11-01), 9049-9060 |x 0175-7598 |q 99:21<9049 |1 2015 |2 99 |o 253 | ||