Establishment of a functional secretory IgA transcytosis model system in vitro for functional food screening
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6501-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6501-9 | ||
| 245 | 0 | 0 | |a Establishment of a functional secretory IgA transcytosis model system in vitro for functional food screening |h [Elektronische Daten] |c [Tao Zuo, Xianqi Feng, Na Zhang, Changhu Xue, Qing-Juan Tang] |
| 520 | 3 | |a A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney (MDCK) cell line that expresses the human polymeric immunoglobulin receptor (pIgR). We thus isolated the human dIgA (dimeric IgA)/pIgA (polymeric IgA) complex as the binding ligands. In the present study, a recombinant vector encoding the human pIgR gene was constructed and infected into MDCK cells. Following reverse transcription polymerase chain reaction (RT-PCR), immunoblotting, and immunofluorescence staining, we confirmed that pIgR was expressed in the transfectant MDCK-pIgR cells and was located at the basolateral side of the cell surface. We also confirmed the coexistence of the dIgA/pIgA complex in the IgA myeloma serum. The covalent dIgA/pIgA complex was then isolated from the serum of an IgA multiple myeloma patient using an ÄKTA purifier operation system with a HiPrep 16/60 Sephacryl S-300 HR column, in order to utilize the complex as transcytosis ligands for human pIgR. Finally, we confirmed the uptake of the isolated human dIgA/pIgA complex into MDCK-pIgR cells. We demonstrated that the human dIgA/pIgA complex was transcytosed into the apical side of the monolayer cells. Therefore, our MDCK-human pIgR cell transcytosis model is an operational system and can be used for screening functional food components that promote dIgA/pIgR transcytosis as well as SIgA secretion. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a SIgA |2 nationallicence | |
| 690 | 7 | |a pIgR |2 nationallicence | |
| 690 | 7 | |a MDCK |2 nationallicence | |
| 690 | 7 | |a dIgA |2 nationallicence | |
| 690 | 7 | |a pIgA |2 nationallicence | |
| 690 | 7 | |a Transcytosis |2 nationallicence | |
| 700 | 1 | |a Zuo |D Tao |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Feng |D Xianqi |u The Affiliated Hospital of Medical College of Qingdao University, 16 Jiangsu Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhang |D Na |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Xue |D Changhu |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | |
| 700 | 1 | |a Tang |D Qing-Juan |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5535-5545 |x 0175-7598 |q 99:13<5535 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6501-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6501-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zuo |D Tao |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Feng |D Xianqi |u The Affiliated Hospital of Medical College of Qingdao University, 16 Jiangsu Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Na |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xue |D Changhu |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tang |D Qing-Juan |u College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5535-5545 |x 0175-7598 |q 99:13<5535 |1 2015 |2 99 |o 253 | ||