caa a22 4500 605505888 CHVBK 20210128100624.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6626-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6626-x Identification of mannitol as compatible solute in Gluconobacter oxydans [Elektronische Daten] [Nageena Zahid, Paul Schweiger, Erwin Galinski, Uwe Deppenmeier] Gluconobacter oxydans is an industrially important bacterium owing to its regio- and enantio-selective incomplete oxidation of various sugars, alcohols, and polyols. The complete genome sequence is available, but it is still unknown how the organism adapts to highly osmotic sugar-rich environments. Therefore, the mechanisms of osmoprotection in G. oxydans were investigated. The accumulation and transport of solutes are hallmarks of osmoadaptation. To identify potential osmoprotectants, G. oxydans was grown on a yeast glucose medium in the presence of 100mM potassium phosphate (pH 7.0) along with various concentrations of sucrose (0-600mM final concentration), which was not metabolized. Intracellular metabolites were analyzed by HPLC and 13C NMR spectroscopy under stress conditions. Both of these analytical techniques highlighted the accumulation of mannitol as a potent osmoprotectant inside the stressed cells. This intracellular mannitol accumulation correlated with increased extracellular osmolarity of the medium. For further confirmation, the growth behavior of G. oxydans was analyzed in the presence of small amounts of mannitol (2.5-10mM) and 300mM sucrose. Growth under sucrose-induced osmotic stress conditions was almost identical to control growth when exogenous mannitol was added in low amounts. Thus, mannitol alleviates the osmotic stress of sucrose on cellular growth. Moreover, the positive effect of exogenous mannitol on the rate of glucose consumption and gluconate formation was also monitored. These results may be helpful to optimize the processes of industrial product formation in highly concentrated sugar solutions. Springer-Verlag Berlin Heidelberg, 2015 Acetic acid bacteria nationallicence Osmotic stress nationallicence Osmoprotection nationallicence Glucose dehydrogenase nationallicence Membrane protein nationallicence Biotransformation nationallicence Zahid Nageena Institute of Microbiology and Biotechnology, Meckenheimer Allee 168, 53115, Bonn, Germany aut Schweiger Paul Biology Department, Missouri State University, 901 S. National Ave, 65897, Springfield, MO, USA aut Galinski Erwin Institute of Microbiology and Biotechnology, Meckenheimer Allee 168, 53115, Bonn, Germany aut Deppenmeier Uwe Institute of Microbiology and Biotechnology, Meckenheimer Allee 168, 53115, Bonn, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5511-5521 0175-7598 99:13<5511 2015 99 253 https://doi.org/10.1007/s00253-015-6626-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6626-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zahid Nageena Institute of Microbiology and Biotechnology, Meckenheimer Allee 168, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Schweiger Paul Biology Department, Missouri State University, 901 S. National Ave, 65897, Springfield, MO, USA aut NATIONALLICENCE 700 1- Galinski Erwin Institute of Microbiology and Biotechnology, Meckenheimer Allee 168, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Deppenmeier Uwe Institute of Microbiology and Biotechnology, Meckenheimer Allee 168, 53115, Bonn, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5511-5521 0175-7598 99:13<5511 2015 99 253