caa a22 4500 605505896 CHVBK 20210128100624.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6540-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6540-2 Glucose consumption rate critically depends on redox state in Corynebacterium glutamicum under oxygen deprivation [Elektronische Daten] [Yota Tsuge, Kimio Uematsu, Shogo Yamamoto, Masako Suda, Hideaki Yukawa, Masayuki Inui] Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose consumption rate and intracellular NADH/NAD+ ratio in various mutant strains. The NADH/NAD+ ratio was strongly repressed under oxygen deprivation when glucose consumption was accelerated by the addition of pyruvate or sodium hydrogen carbonate. Overexpression of the ndh gene in the wild-type strain under oxygen deprivation decreased the NADH/NAD+ ratio from 0.32 to 0.13, whereas the glucose consumption rate increased by 27%. Similarly, in phosphoenolpyruvate carboxylase gene (ppc)- or malate dehydrogenase gene (mdh)-deficient strains, overexpression of the ndh gene decreased the NADH/NAD+ ratio from 1.66 to 0.37 and 2.20 to 0.57, respectively, whereas the glucose consumption rate increased by 57 and 330%, respectively. However, in a lactate dehydrogenase gene (L-ldhA)-deficient strain, although the NADH/NAD+ ratio decreased from 5.62 to 1.13, the glucose consumption rate was not markedly altered. In a tailored d-lactate-producing strain, which lacked ppc and L-ldhA genes, but expressed D-ldhA from Lactobacillus delbrueckii, overexpression of the ndh gene decreased the NADH/NAD+ ratio from 1.77 to 0.56, and increased the glucose consumption rate by 50%. Overall, the glucose consumption rate was found to be inversely proportional to the NADH/NAD+ ratio in C. glutamicum cultured under oxygen deprivation. These findings could provide an option to increase the productivity of chemicals and fuels under oxygen deprivation. Springer-Verlag Berlin Heidelberg, 2015 Corynebacterium glutamicum nationallicence Oxygen deprivation nationallicence NADH/NAD+ ratio nationallicence NADH dehydrogenase nationallicence Glucose consumption rate nationallicence Tsuge Yota Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut Uematsu Kimio Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut Yamamoto Shogo Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut Suda Masako Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut Yukawa Hideaki Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut Inui Masayuki Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5573-5582 0175-7598 99:13<5573 2015 99 253 https://doi.org/10.1007/s00253-015-6540-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6540-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsuge Yota Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut NATIONALLICENCE 700 1- Uematsu Kimio Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamamoto Shogo Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut NATIONALLICENCE 700 1- Suda Masako Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut NATIONALLICENCE 700 1- Yukawa Hideaki Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut NATIONALLICENCE 700 1- Inui Masayuki Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, 619-0292, Kizugawa-shi, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5573-5582 0175-7598 99:13<5573 2015 99 253