caa a22 4500 605505918 CHVBK 20210128100624.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6667-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6667-1 Domestic wastewater treatment in a novel sequencing batch biofilm filter [Elektronische Daten] [Bin Ji, Li Wei, Dan Chen, Hongyu Wang, Zhenhua Li, Kai Yang] Biological treatment of domestic sewage low C/N ratio was accomplished in a pilot-scale sequencing batch biofilm filter (SBBF). The novel hybrid bioreactor consisted of bio-band in the upper and anthracite filter media in the bottom, which combined a sequencing batch biofilm reactor (SBBR) with a biological filter. The average removal efficiency values of chemical oxygen demand (COD), ammonium nitrogen (NH4 +-N), total nitrogen (TN), and total phosphorus (TP) were 89.4, 83.3, 62.9, and 48.7%, respectively. A 454-pyrosequencing technology was employed to investigate the microbial communities of the influent (J1) and the biofilm (J2) on the bio-band on day 40. Pyrosequencing analysis of the 16S rRNA gene revealed the community of the biofilm consisted of Gammaproteobacteria (48.6%), Planctomycetacia (18.0%), Alphaproteobacteria (13.7%), Clostridia (9.6%), Desulfonatronum (18.5%), Actinobacteria (1.9%), and Bacilli (1.7%), accounting for 93.6% of total operational taxonomic units at genera level. Acinetobacter tjernbergiae and Acinetobacter lwoffii were the most abundant species, suggesting that denitrifying phosphorus removal was achieved in the SBBF. Springer-Verlag Berlin Heidelberg, 2015 Wastewater treatment nationallicence Denitrifying phosphorus removal nationallicence Pyrosequencing nationallicence Sequencing batch biofilm filter nationallicence Ji Bin School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Wei Li State Key Lab of Urban Water Resources and Environment, Harbin Institute of Technology, 150090, Harbin, China aut Chen Dan School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Wang Hongyu School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Li Zhenhua Department of Environmental Science and Engineering, College of Resources and Environment, Huazhong Agricultural University, 430072, Wuhan, China aut Yang Kai School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5731-5738 0175-7598 99:13<5731 2015 99 253 https://doi.org/10.1007/s00253-015-6667-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6667-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ji Bin School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Wei Li State Key Lab of Urban Water Resources and Environment, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Chen Dan School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Wang Hongyu School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Li Zhenhua Department of Environmental Science and Engineering, College of Resources and Environment, Huazhong Agricultural University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Yang Kai School of Civil Engineering, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5731-5738 0175-7598 99:13<5731 2015 99 253