Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS
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| 003 | CHVBK | ||
| 005 | 20210128100624.0 | ||
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| 008 | 210128e20150701xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6515-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6515-3 | ||
| 245 | 0 | 0 | |a Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS |h [Elektronische Daten] |c [Dominik Ziegler, Joël Pothier, Julie Ardley, Romain Fossou, Valentin Pflüger, Sofie de Meyer, Guido Vogel, Mauro Tonolla, John Howieson, Wayne Reeve, Xavier Perret] |
| 520 | 3 | |a Accurate identification of soil bacteria that form nitrogen-fixing associations with legume crops is challenging given the phylogenetic diversity of root nodule bacteria (RNB). The labor-intensive and time-consuming 16S ribosomal RNA (rRNA) sequencing and/or multilocus sequence analysis (MLSA) of conserved genes so far remain the favored molecular tools to characterize symbiotic bacteria. With the development of mass spectrometry (MS) as an alternative method to rapidly identify bacterial isolates, we recently showed that matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) can accurately characterize RNB found inside plant nodules or grown in cultures. Here, we report on the development of a MALDI-TOF RNB-specific spectral database built on whole cell MS fingerprints of 116 strains representing the major rhizobial genera. In addition to this RNB-specific module, which was successfully tested on unknown field isolates, a subset of 13 ribosomal proteins extracted from genome data was found to be sufficient for the reliable identification of nodule isolates to rhizobial species as shown in the putatively ascribed ribosomal protein masses (PARPM) database. These results reveal that data gathered from genome sequences can be used to expand spectral libraries to aid the accurate identification of bacterial species by MALDI-TOF MS. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Bacterial fingerprints |2 nationallicence | |
| 690 | 7 | |a Phylogeny |2 nationallicence | |
| 690 | 7 | |a Cluster analysis |2 nationallicence | |
| 690 | 7 | |a Rhizobia |2 nationallicence | |
| 690 | 7 | |a Legume nodules |2 nationallicence | |
| 690 | 7 | |a GEBA-RNB |2 nationallicence | |
| 700 | 1 | |a Ziegler |D Dominik |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | |
| 700 | 1 | |a Pothier |D Joël |u Environmental Genomics and Systems Biology Research Group, Institute for Natural Resources Sciences, Zurich University of Applied Sciences (ZHAW), Grüental, P.O. Box, CH-8820, Wädenswil, Switzerland |4 aut | |
| 700 | 1 | |a Ardley |D Julie |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | |
| 700 | 1 | |a Fossou |D Romain |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | |
| 700 | 1 | |a Pflüger |D Valentin |u Mabritec, Lörracherstrasse 50, CH-4125, Riehen, Switzerland |4 aut | |
| 700 | 1 | |a de Meyer |D Sofie |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | |
| 700 | 1 | |a Vogel |D Guido |u Mabritec, Lörracherstrasse 50, CH-4125, Riehen, Switzerland |4 aut | |
| 700 | 1 | |a Tonolla |D Mauro |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | |
| 700 | 1 | |a Howieson |D John |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | |
| 700 | 1 | |a Reeve |D Wayne |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | |
| 700 | 1 | |a Perret |D Xavier |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5547-5562 |x 0175-7598 |q 99:13<5547 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6515-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6515-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ziegler |D Dominik |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pothier |D Joël |u Environmental Genomics and Systems Biology Research Group, Institute for Natural Resources Sciences, Zurich University of Applied Sciences (ZHAW), Grüental, P.O. Box, CH-8820, Wädenswil, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ardley |D Julie |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fossou |D Romain |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pflüger |D Valentin |u Mabritec, Lörracherstrasse 50, CH-4125, Riehen, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a de Meyer |D Sofie |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vogel |D Guido |u Mabritec, Lörracherstrasse 50, CH-4125, Riehen, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tonolla |D Mauro |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Howieson |D John |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Reeve |D Wayne |u Center for Rhizobium Studies, Murdoch University, Murdoch, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Perret |D Xavier |u Department of Botany and Plant Biology, Microbiology Unit, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5547-5562 |x 0175-7598 |q 99:13<5547 |1 2015 |2 99 |o 253 | ||