caa a22 4500 605505969 CHVBK 20210128100624.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6408-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6408-5 Barcoded pyrosequencing-based metagenomic analysis of the faecal microbiome of three purebred pig lines after cohabitation [Elektronische Daten] [Edward Pajarillo, Jong Chae, Hyeun Kim, In Kim, Dae-Kyung Kang] The microbial communities in the pig gut perform a variety of beneficial functions. Along with host genetics and diet, farm management practices are an important aspect of agricultural animal production that could influence gut microbial diversity. In this study, we used barcoded pyrosequencing of the V1-V3 regions of the 16S ribosomal RNA (rRNA) genes to characterise the faecal microbiome of three common commercial purebred pig lines (Duroc, Landrace and Yorkshire) before and after cohabitation. The diversity of faecal microbiota was characterised by employing phylogenetic, distance-based and multivariate-clustering approaches. Bacterial diversity tended to become more uniform after mixing of the litters. Age-related shifts were also observed at various taxonomic levels, with an increase in the proportion of the phylum Firmicutes and a decrease in Bacteroidetes over time, regardless of the purebred group. Cohabitation had a detectable effect on the microbial shift among purebred pigs. We identified the bacterial genus Parasutterella as having utility in discriminating pigs according to time. Similarly, Dialister and Bacteroides can be used to differentiate the purebred lines used. The microbial communities of the three purebred pigs became more similar after cohabitation, but retained a certain degree of breed specificity, with the microbiota of Landrace and Yorkshire remaining distinct from that of their distant relative, Duroc. Springer-Verlag Berlin Heidelberg, 2015 Microbial diversity nationallicence Faecal microbiome nationallicence Pyrosequencing nationallicence 16S rRNA gene nationallicence Cohabitation nationallicence Purebred pigs nationallicence Pajarillo Edward Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut Chae Jong Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut Kim Hyeun Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut Kim In Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut Kang Dae-Kyung Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5647-5656 0175-7598 99:13<5647 2015 99 253 https://doi.org/10.1007/s00253-015-6408-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6408-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pajarillo Edward Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut NATIONALLICENCE 700 1- Chae Jong Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Hyeun Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut NATIONALLICENCE 700 1- Kim In Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut NATIONALLICENCE 700 1- Kang Dae-Kyung Department of Animal Resources Science, Dankook University, 119 Dandae-ro, 330-714, Cheonan, Republic of Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5647-5656 0175-7598 99:13<5647 2015 99 253