In vitro effect of temperature on the conformational structure and collagen binding of SdrF, a Staphylococcus epidermidis adhesin
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6456-x |2 doi |
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| 245 | 0 | 0 | |a In vitro effect of temperature on the conformational structure and collagen binding of SdrF, a Staphylococcus epidermidis adhesin |h [Elektronische Daten] |c [Antonella Di Poto, Massimiliano Papi, Sheetal Trivedi, Alessandro Maiorana, Paola Gavazzo, Massimo Vassalli, Franklin Lowy, Marco De Spirito, Lucio Montanaro, Marcello Imbriani, Carla Arciola, Livia Visai] |
| 520 | 3 | |a Staphylococcus epidermidis is the leading etiologic agent of device-related infections. S. epidermidis is able to bind, by means of the adhesins of its cell wall, the host matrix proteins filming the artificial surfaces. Thence, bacteria cling to biomaterials and infection develops. The effect of temperature on integrity, structure, and biological activity of the collagen-binding adhesin (SdrF) of S. epidermidis has been here investigated. By cloning in E. coli XL1-Blue, a recombinant of the SdrF binding domain B (rSdrFB), carrying an N-terminal polyhistidine, was obtained. Purification was by HiTrapTM Chelating HP columns. Assessment of purity, molecular weight, and integrity was by SDS-PAGE. The rSdrFB-collagen binding was investigated by ELISA. A full three-dimensional reconstruction of rSdrFB was achieved by small-angle X-ray scattering (SAXS). At 25°C, rSdrFB bound to type I collagen in a dose-dependent, saturable manner, with a Kd of 2.48 × 10−7M. When temperature increased from 25 to 37°C, a strong conformational change occurred, together with the abolition of the rSdrFB-collagen binding. The rSdrFB integrity was not affected by temperature variation. SdrFB-collagen binding is switched on/off depending on the temperature. Implications with the infection pathogenesis are enlightened. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Staphylococcus epidermidis |2 nationallicence | |
| 690 | 7 | |a SdrF adhesin |2 nationallicence | |
| 690 | 7 | |a Collagen |2 nationallicence | |
| 690 | 7 | |a Small-angle X-ray scattering (SAXS) |2 nationallicence | |
| 690 | 7 | |a Normalized spatial discrepancies (NSD) |2 nationallicence | |
| 690 | 7 | |a Hypothermia |2 nationallicence | |
| 690 | 7 | |a Opportunistic infections |2 nationallicence | |
| 700 | 1 | |a Di Poto |D Antonella |u Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy |4 aut | |
| 700 | 1 | |a Papi |D Massimiliano |u Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy |4 aut | |
| 700 | 1 | |a Trivedi |D Sheetal |u Division of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street Box 82, 10032, New York, NY, USA |4 aut | |
| 700 | 1 | |a Maiorana |D Alessandro |u Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy |4 aut | |
| 700 | 1 | |a Gavazzo |D Paola |u Institute of Biophysics of the National Research Council of Italy, Via De Marini 6, 16149, Genoa, Italy |4 aut | |
| 700 | 1 | |a Vassalli |D Massimo |u Institute of Biophysics of the National Research Council of Italy, Via De Marini 6, 16149, Genoa, Italy |4 aut | |
| 700 | 1 | |a Lowy |D Franklin |u Division of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street Box 82, 10032, New York, NY, USA |4 aut | |
| 700 | 1 | |a De Spirito |D Marco |u Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy |4 aut | |
| 700 | 1 | |a Montanaro |D Lucio |u Research Unit on Implant Infections, Rizzoli Orthopedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | |
| 700 | 1 | |a Imbriani |D Marcello |u Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Via Forlanini 2, 27100, Pavia, Italy |4 aut | |
| 700 | 1 | |a Arciola |D Carla |u Research Unit on Implant Infections, Rizzoli Orthopedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | |
| 700 | 1 | |a Visai |D Livia |u Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5593-5603 |x 0175-7598 |q 99:13<5593 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6456-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6456-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Di Poto |D Antonella |u Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Papi |D Massimiliano |u Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Trivedi |D Sheetal |u Division of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street Box 82, 10032, New York, NY, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Maiorana |D Alessandro |u Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gavazzo |D Paola |u Institute of Biophysics of the National Research Council of Italy, Via De Marini 6, 16149, Genoa, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vassalli |D Massimo |u Institute of Biophysics of the National Research Council of Italy, Via De Marini 6, 16149, Genoa, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lowy |D Franklin |u Division of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street Box 82, 10032, New York, NY, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a De Spirito |D Marco |u Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Montanaro |D Lucio |u Research Unit on Implant Infections, Rizzoli Orthopedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Imbriani |D Marcello |u Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Via Forlanini 2, 27100, Pavia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Arciola |D Carla |u Research Unit on Implant Infections, Rizzoli Orthopedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Visai |D Livia |u Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5593-5603 |x 0175-7598 |q 99:13<5593 |1 2015 |2 99 |o 253 | ||