caa a22 4500 605505993 CHVBK 20210128100624.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6454-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6454-z Occurrence and importance of anaerobic ammonium-oxidising bacteria in vegetable soils [Elektronische Daten] [Li-dong Shen, Hong-sheng Wu, Zhi-qiu Gao, Xiang-hua Xu, Tie-xi Chen, Shuai Liu, Hai-xiang Cheng] The quantitative importance of anaerobic ammonium oxidation (anammox) has been described in paddy fields, while the presence and importance of anammox in subsurfacesoil from vegetable fields have not been determined yet. Here, we investigated the occurrence and activity of anammox bacteria in five different types of vegetable fields located in Jiangsu Province, China. Stable isotope experiments confirmed the anammox activity in the examined soils, with the potential rates of 2.1 and 23.2nmolN2 g−1 dry soil day−1, and the anammox accounted for 5.9-20.5% of total soil dinitrogen gas production. It is estimated that a total loss of 7.1-78.2gNm−2year−1 could be linked to the anammox process in the examined vegetable fields. Phylogenetic analyses showed that multiple co-occurring anammox genera were present in the examined soils, including Candidatus Brocadia, Candidatus Kuenenia, Candidatus Anammoxoglobus and Candidatus Jettenia, and Candidatus Brocadia appeared to be the most common anammox genus. Quantitative PCR further confirmed the presence of anammox bacteria in the examined soils, with the abundance varying from 2.8 × 105 to 3.0 × 106 copies g−1 dry soil. Correlation analyses suggested that the soil ammonium concentration had significant influence on the activity and abundance of anammox bacteria in the examined soils. The results of our study showed the presence of diverse anammox bacteria and indicated that the anammox process could serve as an important nitrogen loss pathway in vegetable fields. Springer-Verlag Berlin Heidelberg, 2015 Anammox nationallicence Abundance nationallicence Activity nationallicence Nitrogen loss nationallicence Vegetable fields nationallicence Agricultural soils nationallicence Shen Li-dong Jiangsu Key Laboratory of Agricultural Meteorology, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, College of Applied Meteorology, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut Wu Hong-sheng Jiangsu Key Laboratory of Agricultural Meteorology, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, College of Applied Meteorology, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut Gao Zhi-qiu State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Science, Beijing, China aut Xu Xiang-hua Jiangsu Key Laboratory of Agricultural Meteorology, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, College of Applied Meteorology, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut Chen Tie-xi College of Geophysics and Remote Sensing, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut Liu Shuai Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Cheng Hai-xiang College of Chemistry and Materials Engineering, Quzhou University, 324000, Quzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5709-5718 0175-7598 99:13<5709 2015 99 253 https://doi.org/10.1007/s00253-015-6454-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6454-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Li-dong Jiangsu Key Laboratory of Agricultural Meteorology, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, College of Applied Meteorology, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut NATIONALLICENCE 700 1- Wu Hong-sheng Jiangsu Key Laboratory of Agricultural Meteorology, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, College of Applied Meteorology, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut NATIONALLICENCE 700 1- Gao Zhi-qiu State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Science, Beijing, China aut NATIONALLICENCE 700 1- Xu Xiang-hua Jiangsu Key Laboratory of Agricultural Meteorology, Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, College of Applied Meteorology, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut NATIONALLICENCE 700 1- Chen Tie-xi College of Geophysics and Remote Sensing, Nanjing University of Information Science and Technology, 210044, Nanjing, China aut NATIONALLICENCE 700 1- Liu Shuai Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Cheng Hai-xiang College of Chemistry and Materials Engineering, Quzhou University, 324000, Quzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5709-5718 0175-7598 99:13<5709 2015 99 253