Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605506035 | ||
| 003 | CHVBK | ||
| 005 | 20210128100625.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150701xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6678-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6678-y | ||
| 245 | 0 | 0 | |a Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis |h [Elektronische Daten] |c [Xue Zhang, Chong Zhang, Qian-Qian Zhou, Xiao-Fei Zhang, Li-Yan Wang, Hai-Bo Chang, He-Ping Li, Yoshimitsu Oda, Xin-Hui Xing] |
| 520 | 3 | |a DNA damage is the dominant source of mutation, which is the driving force of evolution. Therefore, it is important to quantitatively analyze the DNA damage caused by different mutagenesis methods, the subsequent mutation rates, and their relationship. Atmospheric and room temperature plasma (ARTP) mutagenesis has been used for the mutation breeding of more than 40 microorganisms. However, ARTP mutagenesis has not been quantitatively compared with conventional mutation methods. In this study, the umu test using a flow-cytometric analysis was developed to quantify the DNA damage in individual viable cells using Salmonella typhimurium NM2009 as the model strain and to determine the mutation rate. The newly developed method was used to evaluate four different mutagenesis systems: a new ARTP tool, ultraviolet radiation, 4-nitroquinoline-1-oxide (4-NQO), and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) mutagenesis. The mutation rate was proportional to the corresponding SOS response induced by DNA damage. ARTP caused greater DNA damage to individual living cells than the other conventional mutagenesis methods, and the mutation rate was also higher. By quantitatively comparing the DNA damage and consequent mutation rate after different types of mutagenesis, we have shown that ARTP is a potentially powerful mutagenesis tool with which to improve the characteristics of microbial cell factories. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a ARTP mutagenesis |2 nationallicence | |
| 690 | 7 | |a Flow cytometry |2 nationallicence | |
| 690 | 7 | |a Fluctuation test |2 nationallicence | |
| 690 | 7 | |a umu test |2 nationallicence | |
| 690 | 7 | |a SOS response |2 nationallicence | |
| 700 | 1 | |a Zhang |D Xue |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | |
| 700 | 1 | |a Zhang |D Chong |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | |
| 700 | 1 | |a Zhou |D Qian-Qian |u Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China |4 aut | |
| 700 | 1 | |a Zhang |D Xiao-Fei |u Department of Engineering Physics, Tsinghua University, Beijing, China |4 aut | |
| 700 | 1 | |a Wang |D Li-Yan |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | |
| 700 | 1 | |a Chang |D Hai-Bo |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | |
| 700 | 1 | |a Li |D He-Ping |u Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China |4 aut | |
| 700 | 1 | |a Oda |D Yoshimitsu |u Institute of Life and Environmental Sciences, Osaka Shin-Ai College, Tsurumi, Tsurumi-ku, Osaka, Japan |4 aut | |
| 700 | 1 | |a Xing |D Xin-Hui |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5639-5646 |x 0175-7598 |q 99:13<5639 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6678-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6678-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Xue |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Chong |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Qian-Qian |u Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Xiao-Fei |u Department of Engineering Physics, Tsinghua University, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Li-Yan |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chang |D Hai-Bo |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D He-Ping |u Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oda |D Yoshimitsu |u Institute of Life and Environmental Sciences, Osaka Shin-Ai College, Tsurumi, Tsurumi-ku, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xing |D Xin-Hui |u Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5639-5646 |x 0175-7598 |q 99:13<5639 |1 2015 |2 99 |o 253 | ||