caa a22 4500 605506035 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6678-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6678-y Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis [Elektronische Daten] [Xue Zhang, Chong Zhang, Qian-Qian Zhou, Xiao-Fei Zhang, Li-Yan Wang, Hai-Bo Chang, He-Ping Li, Yoshimitsu Oda, Xin-Hui Xing] DNA damage is the dominant source of mutation, which is the driving force of evolution. Therefore, it is important to quantitatively analyze the DNA damage caused by different mutagenesis methods, the subsequent mutation rates, and their relationship. Atmospheric and room temperature plasma (ARTP) mutagenesis has been used for the mutation breeding of more than 40 microorganisms. However, ARTP mutagenesis has not been quantitatively compared with conventional mutation methods. In this study, the umu test using a flow-cytometric analysis was developed to quantify the DNA damage in individual viable cells using Salmonella typhimurium NM2009 as the model strain and to determine the mutation rate. The newly developed method was used to evaluate four different mutagenesis systems: a new ARTP tool, ultraviolet radiation, 4-nitroquinoline-1-oxide (4-NQO), and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) mutagenesis. The mutation rate was proportional to the corresponding SOS response induced by DNA damage. ARTP caused greater DNA damage to individual living cells than the other conventional mutagenesis methods, and the mutation rate was also higher. By quantitatively comparing the DNA damage and consequent mutation rate after different types of mutagenesis, we have shown that ARTP is a potentially powerful mutagenesis tool with which to improve the characteristics of microbial cell factories. Springer-Verlag Berlin Heidelberg, 2015 ARTP mutagenesis nationallicence Flow cytometry nationallicence Fluctuation test nationallicence umu test nationallicence SOS response nationallicence Zhang Xue Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut Zhang Chong Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut Zhou Qian-Qian Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China aut Zhang Xiao-Fei Department of Engineering Physics, Tsinghua University, Beijing, China aut Wang Li-Yan Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut Chang Hai-Bo Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut Li He-Ping Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China aut Oda Yoshimitsu Institute of Life and Environmental Sciences, Osaka Shin-Ai College, Tsurumi, Tsurumi-ku, Osaka, Japan aut Xing Xin-Hui Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5639-5646 0175-7598 99:13<5639 2015 99 253 https://doi.org/10.1007/s00253-015-6678-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6678-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xue Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut NATIONALLICENCE 700 1- Zhang Chong Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut NATIONALLICENCE 700 1- Zhou Qian-Qian Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Zhang Xiao-Fei Department of Engineering Physics, Tsinghua University, Beijing, China aut NATIONALLICENCE 700 1- Wang Li-Yan Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut NATIONALLICENCE 700 1- Chang Hai-Bo Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut NATIONALLICENCE 700 1- Li He-Ping Biobreeding Research Center, Wuxi Research Institute of Applied Technologies, Tsinghua University, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Oda Yoshimitsu Institute of Life and Environmental Sciences, Osaka Shin-Ai College, Tsurumi, Tsurumi-ku, Osaka, Japan aut NATIONALLICENCE 700 1- Xing Xin-Hui Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Department of Chemical Engineering, Tsinghua University, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5639-5646 0175-7598 99:13<5639 2015 99 253