caa a22 4500 605506043 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6482-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6482-8 Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect [Elektronische Daten] [I. Presti, G. D'Orazio, M. Labra, B. La Ferla, V. Mezzasalma, G. Bizzaro, S. Giardina, A. Michelotti, F. Tursi, M. Vassallo, P. Di Gennaro] Probiotic ingestion is recommended as a preventive approach to maintain the balance of the intestinal microbiota and to enhance the human well-being. During the whole life of each individual, the gut microbiota composition could be altered by lifestyle, diet, antibiotic therapies and other stress conditions, which may lead to acute and chronic disorders. Hence, probiotics can be administered for the prevention or treatment of some disorders, including lactose malabsorption, acute diarrhoea, irritable bowel syndrome, necrotizing enterocolitis and mild forms of inflammatory bowel disease. The probiotic-mediated effect is an important issue that needs to be addressed in relation to strain-specific probiotic properties. In this work, the probiotic properties of new Lactobacillus and Bifidobacterium strains were screened, and their effects in vitro were evaluated. They were screened for probiotic properties by determining their tolerance to low pH and to bile salts, antibiotic sensitivity, antimicrobial activity and vitamin B8, B9 and B12 production, and by considering their ability to increase the antioxidant potential and to modulate the inflammatory status of systemic-miming cell lines in vitro. Three out of the examined strains presenting the most performant probiotic properties, as Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070 and Bifidobacterium animalis subsp. lactis PBSO75, were evaluated for their effects also on human intestinal HT-29 cell line. The obtained results support the possibility to move to another level of study, that is, the oral administration of these probiotical strains to patients with acute and chronic gut disorders, by in vivo experiments. Springer-Verlag Berlin Heidelberg, 2015 Probiotics nationallicence Lactobacillus nationallicence Bifidobacterium nationallicence Intestinal microbiota nationallicence Gut disorders nationallicence Presti I. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut D'Orazio G. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut Labra M. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut La Ferla B. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut Mezzasalma V. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut Bizzaro G. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut Giardina S. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut Michelotti A. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut Tursi F. Farcos Srl, Garbagnate Milanese, Milano, Italy aut Vassallo M. Farcos Srl, Garbagnate Milanese, Milano, Italy aut Di Gennaro P. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5613-5626 0175-7598 99:13<5613 2015 99 253 https://doi.org/10.1007/s00253-015-6482-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6482-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Presti I. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut NATIONALLICENCE 700 1- D'Orazio G. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut NATIONALLICENCE 700 1- Labra M. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut NATIONALLICENCE 700 1- La Ferla B. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut NATIONALLICENCE 700 1- Mezzasalma V. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut NATIONALLICENCE 700 1- Bizzaro G. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut NATIONALLICENCE 700 1- Giardina S. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut NATIONALLICENCE 700 1- Michelotti A. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut NATIONALLICENCE 700 1- Tursi F. Farcos Srl, Garbagnate Milanese, Milano, Italy aut NATIONALLICENCE 700 1- Vassallo M. Farcos Srl, Garbagnate Milanese, Milano, Italy aut NATIONALLICENCE 700 1- Di Gennaro P. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milano, Italy aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5613-5626 0175-7598 99:13<5613 2015 99 253