Characterization of an omega-6 linoleate lipoxygenase from Burkholderia thailandensis and its application in the production of 13-hydroxyoctadecadienoic acid
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6353-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6353-8 | ||
| 245 | 0 | 0 | |a Characterization of an omega-6 linoleate lipoxygenase from Burkholderia thailandensis and its application in the production of 13-hydroxyoctadecadienoic acid |h [Elektronische Daten] |c [Jung-Ung An, Baek-Joong Kim, Seung-Hye Hong, Deok-Kun Oh] |
| 520 | 3 | |a A recombinant putative lipoxygenase from Burkholderia thailandensis with a specific activity of 26.4Umg−1 was purified using HisTrap affinity chromatography. The native enzyme was a 75-kDa dimer with a molecular mass of 150kDa. The enzyme activity and catalytic efficiency (k cat/K m) were the highest for linoleic acid (k cat of 93.7s−1 and K m of 41.5μM), followed by arachidonic acid, α-linolenic acid, and γ-linolenic acid. The enzyme was identified as an omega-6 linoleate lipoxygenase (or a linoleate 13S-lipoxygenase) based on genetic and HPLC analyses as well as substrate specificity. The reaction conditions for the enzymatic production of 13-hydroxy-9,11(Z,E)-octadecadienoic acid (13-HODE) were optimal at pH 7.5, 25°C, 20gl−1 linoleic acid, 2.5gl−1 enzyme, 0.1mM Cu2+, and 6% (v/v) methanol. Under these conditions, linoleate 13-lipoxygenase from B. thailandensis produced 20.8gl−1 13-HODE (70.2mM) from 20gl−1 linoleic acid (71.3mM) for 120min, with a molar conversion yield of 98.5% and productivity of 10.4gl−1h−1. The molar conversion yield and productivity of 13-HODE obtained using B. thailandensis lipoxygenase were 151 and 158% higher, respectively, than those obtained using commercial soybean lipoxygenase under the optimum conditions for each enzyme at the same concentrations of substrate and enzyme. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Burkholderia thailandensis |2 nationallicence | |
| 690 | 7 | |a 13-Hydroxyoctadecadienoic acid |2 nationallicence | |
| 690 | 7 | |a 13-Hydroperoxyctadecadienoic acid |2 nationallicence | |
| 690 | 7 | |a Linoleate 13-Lipoxygenase |2 nationallicence | |
| 690 | 7 | |a Linoleic acid |2 nationallicence | |
| 700 | 1 | |a An |D Jung-Ung |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Kim |D Baek-Joong |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Hong |D Seung-Hye |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | |
| 700 | 1 | |a Oh |D Deok-Kun |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5487-5497 |x 0175-7598 |q 99:13<5487 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6353-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6353-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a An |D Jung-Ung |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Baek-Joong |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hong |D Seung-Hye |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oh |D Deok-Kun |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, Republic of Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5487-5497 |x 0175-7598 |q 99:13<5487 |1 2015 |2 99 |o 253 | ||