Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6339-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6339-6 | ||
| 245 | 0 | 0 | |a Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice |h [Elektronische Daten] |c [Qingfeng Guan, Xiao Wang, Xiumin Wang, Da Teng, Ruoyu Mao, Yong Zhang, Jianhua Wang] |
| 520 | 3 | |a Pathogenic Escherichia coli (E. coli) is an important infectious Gram-negative bacterium causing millions of death every year. Outer membrane protein A (OmpA) has been suggested as a potential vaccine candidate for conferring protection against bacterial infection. In this study, a universal vaccine candidate for E. coli infection was developed and evaluated. Bioinformatics analysis revealed the OmpA protein from E. coli shares 96~100%, 90~94%, and 45% identity with Shigella, Salmonella, and Pseudomonas strains, respectively. The ompA gene was cloned from the genomic DNA of E. coli, and then the OmpA protein was expressed in BL21 (DE3) using the auto-induction method. The recombinant OmpA (rOmpA) protein had an average molecular weight of 36kDa with the purity of 93.5%. Immunological analysis indicated that the titers of anti-rOmpA sera against rOmpA and whole cells were 1:642,000 and 1:140,000, respectively. Moreover, rOmpA not only conferred a high level of immunogenicity to protect mice against the challenge of E. coli, but also generated cross-protection against Shigella and Salmonella. The anti-rOmpA sera could enhance the phagocytic activity of neutrophils against E. coli. The survive ratios of mice immunized with rOmpA and PBS were 50 %and 20% after 48h post-challenge, indicating mice were protected from E. coli infection after immunization with rOmpA. All these results clearly indicate that rOmpA may be a promising candidate for the development of a subunit vaccine to prevent E. coli infection. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Outer membrane protein A |2 nationallicence | |
| 690 | 7 | |a Immunogenicity |2 nationallicence | |
| 690 | 7 | |a Vaccine |2 nationallicence | |
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 690 | 7 | |a Mice |2 nationallicence | |
| 700 | 1 | |a Guan |D Qingfeng |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Xiao |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Xiumin |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Teng |D Da |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Mao |D Ruoyu |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhang |D Yong |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Jianhua |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5451-5460 |x 0175-7598 |q 99:13<5451 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6339-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6339-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guan |D Qingfeng |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Xiao |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Xiumin |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Teng |D Da |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mao |D Ruoyu |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Yong |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Jianhua |u Key Laboratory of Feed Biotechnology, Ministry of Agriculture, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5451-5460 |x 0175-7598 |q 99:13<5451 |1 2015 |2 99 |o 253 | ||