caa a22 4500 605506078 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6420-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6420-9 Biostimulation of petroleum-hydrocarbon-contaminated marine sediment with co-substrate: involved metabolic process and microbial community [Elektronische Daten] [Zhen Zhang, Irene Lo] This study investigated the effect of acetate and methanol as co-substrates on anaerobic biodegradation of total petroleum hydrocarbons (TPHs, C10-C40) in marine sediment. The findings evidenced that the degradation of TPH can be enhanced by adding acetate or methanol. The addition of acetate was generally more favorable than the addition of methanol for the TPH degradation. Both sulfate reduction and methanogenesis occurred in the acetate-treated sediment. However, the depletion of SO4 2− inhibited sulfate reduction over the incubation period. Only methanogenesis was prevalent in the methanol-treated sediment within the whole incubation period. The degradation of TPH fractions with higher carbon number ranges (C31-C40) was speculated to be more favored under sulfate-reducing condition, while TPH fractions with lower carbon number ranges (C10-C20) were preferentially degraded under methanogenic condition. The 16S rRNA clone library-based analysis revealed that the addition of different co-substrates led to distinct structures of the microbial community. Clones related to sulfate-reducing Desulfobacterales were the most abundant in the sediment dosed with acetate. Clones related to Clostridiales predominated in the sediment dosed with methanol. Acetoclastic methanogens were found to be the predominant archaeal species in the sediment dosed with acetate, while both acetoclastic methanogens and hydrogenotrophic methanogens accounted for large proportions in the sediment dosed with methanol. The results obtained in this study will contribute to more comprehensive knowledge on the role of acetate and methanol as co-substrates in biostimulation of petroleum-hydrocarbon-contaminated marine sediment. Springer-Verlag Berlin Heidelberg, 2015 Biostimulation nationallicence Co-substrate nationallicence Metabolic process nationallicence Microbial community nationallicence Total petroleum hydrocarbons (TPHs) nationallicence Zhang Zhen Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Hong Kong, China aut Lo Irene Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Hong Kong, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5683-5696 0175-7598 99:13<5683 2015 99 253 https://doi.org/10.1007/s00253-015-6420-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6420-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Zhen Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Hong Kong, China aut NATIONALLICENCE 700 1- Lo Irene Department of Civil and Environmental Engineering, The Hong Kong University of Science and Technology, Hong Kong, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5683-5696 0175-7598 99:13<5683 2015 99 253