Analysis of microbial community structure and composition in leachates from a young landfill by 454 pyrosequencing
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6409-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6409-4 | ||
| 245 | 0 | 0 | |a Analysis of microbial community structure and composition in leachates from a young landfill by 454 pyrosequencing |h [Elektronische Daten] |c [Thorsten Köchling, José Sanz, Sávia Gavazza, Lourdinha Florencio] |
| 520 | 3 | |a Microorganisms are responsible for degrading the raw leachate generated in sanitary landfills, extracting the soluble fraction of the landfill waste and biotransforming organic matter and toxic residues. To increase our understanding of these highly contaminated ecosystems, we analyzed the microbial communities in the leachate produced by three landfill cells of different ages. Using high-throughput 454 pyrosequencing of the 16S rRNA gene, we describe the structure of the leachate communities and present their compositional characteristics. All three communities exhibited a high level of abundance but were undersampled, as indicated by the results of the rarefaction analysis. The distribution of the taxonomic operational units (OTUs) was highly skewed, suggesting a community structure with a few dominant members that are key for the degradation process and numerous rare microorganisms, which could act as a resilient microorganism seeder pool. Members of the phylum Firmicutes were dominant in all of the samples, accounting for up to 62% of the bacterial sequences, and their proportion increased with increasing landfill age. Other abundant phyla included Bacteroidetes, Proteobacteria, and Spirochaetes, which together with Firmicutes comprised 90% of the sequences. The data illustrate a microbial community that degrades organic matter in raw leachate in the early stages, before the methanogenic phase takes place. The genera found fit well into the classical pathways of anaerobic digestion processes. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Landfill leachate |2 nationallicence | |
| 690 | 7 | |a 16S rRNA gene |2 nationallicence | |
| 690 | 7 | |a 454 pyrosequencing |2 nationallicence | |
| 690 | 7 | |a Microbial community structure |2 nationallicence | |
| 700 | 1 | |a Köchling |D Thorsten |u Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil |4 aut | |
| 700 | 1 | |a Sanz |D José |u Department of Molecular Biology, Autonomous University of Madrid, 28049, Cantoblanco, Spain |4 aut | |
| 700 | 1 | |a Gavazza |D Sávia |u Laboratory of Environmental Engineering, Federal University of Pernambuco, Academic Center of the Agreste, Rodovia BR-104, Km 62, Nova Caruaru, 55.002-970, Caruaru, PE, Brazil |4 aut | |
| 700 | 1 | |a Florencio |D Lourdinha |u Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5657-5668 |x 0175-7598 |q 99:13<5657 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6409-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6409-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Köchling |D Thorsten |u Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sanz |D José |u Department of Molecular Biology, Autonomous University of Madrid, 28049, Cantoblanco, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gavazza |D Sávia |u Laboratory of Environmental Engineering, Federal University of Pernambuco, Academic Center of the Agreste, Rodovia BR-104, Km 62, Nova Caruaru, 55.002-970, Caruaru, PE, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Florencio |D Lourdinha |u Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5657-5668 |x 0175-7598 |q 99:13<5657 |1 2015 |2 99 |o 253 | ||