caa a22 4500 605506086 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6409-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6409-4 Analysis of microbial community structure and composition in leachates from a young landfill by 454 pyrosequencing [Elektronische Daten] [Thorsten Köchling, José Sanz, Sávia Gavazza, Lourdinha Florencio] Microorganisms are responsible for degrading the raw leachate generated in sanitary landfills, extracting the soluble fraction of the landfill waste and biotransforming organic matter and toxic residues. To increase our understanding of these highly contaminated ecosystems, we analyzed the microbial communities in the leachate produced by three landfill cells of different ages. Using high-throughput 454 pyrosequencing of the 16S rRNA gene, we describe the structure of the leachate communities and present their compositional characteristics. All three communities exhibited a high level of abundance but were undersampled, as indicated by the results of the rarefaction analysis. The distribution of the taxonomic operational units (OTUs) was highly skewed, suggesting a community structure with a few dominant members that are key for the degradation process and numerous rare microorganisms, which could act as a resilient microorganism seeder pool. Members of the phylum Firmicutes were dominant in all of the samples, accounting for up to 62% of the bacterial sequences, and their proportion increased with increasing landfill age. Other abundant phyla included Bacteroidetes, Proteobacteria, and Spirochaetes, which together with Firmicutes comprised 90% of the sequences. The data illustrate a microbial community that degrades organic matter in raw leachate in the early stages, before the methanogenic phase takes place. The genera found fit well into the classical pathways of anaerobic digestion processes. Springer-Verlag Berlin Heidelberg, 2015 Landfill leachate nationallicence 16S rRNA gene nationallicence 454 pyrosequencing nationallicence Microbial community structure nationallicence Köchling Thorsten Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil aut Sanz José Department of Molecular Biology, Autonomous University of Madrid, 28049, Cantoblanco, Spain aut Gavazza Sávia Laboratory of Environmental Engineering, Federal University of Pernambuco, Academic Center of the Agreste, Rodovia BR-104, Km 62, Nova Caruaru, 55.002-970, Caruaru, PE, Brazil aut Florencio Lourdinha Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5657-5668 0175-7598 99:13<5657 2015 99 253 https://doi.org/10.1007/s00253-015-6409-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6409-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Köchling Thorsten Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Sanz José Department of Molecular Biology, Autonomous University of Madrid, 28049, Cantoblanco, Spain aut NATIONALLICENCE 700 1- Gavazza Sávia Laboratory of Environmental Engineering, Federal University of Pernambuco, Academic Center of the Agreste, Rodovia BR-104, Km 62, Nova Caruaru, 55.002-970, Caruaru, PE, Brazil aut NATIONALLICENCE 700 1- Florencio Lourdinha Laboratory of Environmental Sanitation, Department of Civil Engineering, Federal University of Pernambuco, Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária, 50.740-530, Recife, PE, Brazil aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5657-5668 0175-7598 99:13<5657 2015 99 253