caa a22 4500 605506094 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6562-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6562-9 Reactance and resistance: main properties to follow the cell differentiation process in Bacillus thuringiensis by dielectric spectroscopy in real time [Elektronische Daten] [Jabel Dinorín-Téllez-Girón, Raúl Delgado-Macuil, Claudia Larralde Corona, Francisco Martínez Montes, Mayra de la Torre Martínez, Víctor López-Y-López] During growth, Bacillus thuringiensis presents three phases: exponential phase (EP), transition state (TS), and sporulation phase (SP). In order to form a dormant spore and to synthesize delta-endotoxins during SP, bacteria must undergo a cellular differentiation process initiated during the TS. Dielectric spectroscopy is a technique that can be utilized for continuous and in situ monitoring of the cellular state. In order to study on-line cell behavior in B. thuringiensis cultures, we conducted a number of batch cultures under different conditions, by scanning 200 frequencies from 42Hz to 5MHz and applying fixed current and voltage of 20mA and 5V DC, respectively. The resulting signals included Impedance (Z), Angle phase (Deg), Voltage (V), Current (I), Conductance (G), Reactance (X), and Resistance (R). Individual raw data relating to observed dielectric property profiles were correlated with the different growth phases established using data from cellular growth, cry1Ac gene expression, and free spores obtained with conventional techniques and fermentation parameters. Based on these correlations, frequencies of 0.1, 0.5, and 1.225MHz were selected for the purpose of measuring dielectric properties in independent batch cultures, at a fixed frequency. X and R manifest more propitious behavior in relation to EP, TS, SP, and spore release, due to particular changes in their signals. Interestingly, these profiles underwent pronounced changes during EP and TS that were not noticed when using conventional methods, but were indicative of the beginning of the B. thuringiensis cell differentiation process. Springer-Verlag Berlin Heidelberg, 2015 Cell differentiation nationallicence Dielectric spectroscopy nationallicence Bacillus thuringiensis nationallicence On-line measuring nationallicence Resistance ( R ) nationallicence Reactance ( X ) nationallicence Dinorín-Téllez-Girón Jabel Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut Delgado-Macuil Raúl Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut Larralde Corona Claudia Centro de Biotecnología Genómica del Instituto Politécnico Nacional, Boulevard del Maestro S/N esq. Elías Piña, Col. Narciso Mendoza, C.P. 88710, Reynosa, Tamaulipas, México aut Martínez Montes Francisco Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut de la Torre Martínez Mayra Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a La Victoria km 0.6, C.P. 83304, Hermosillo, Sonora, México aut López-Y-López Víctor Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5439-5450 0175-7598 99:13<5439 2015 99 253 https://doi.org/10.1007/s00253-015-6562-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6562-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dinorín-Téllez-Girón Jabel Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut NATIONALLICENCE 700 1- Delgado-Macuil Raúl Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut NATIONALLICENCE 700 1- Larralde Corona Claudia Centro de Biotecnología Genómica del Instituto Politécnico Nacional, Boulevard del Maestro S/N esq. Elías Piña, Col. Narciso Mendoza, C.P. 88710, Reynosa, Tamaulipas, México aut NATIONALLICENCE 700 1- Martínez Montes Francisco Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut NATIONALLICENCE 700 1- de la Torre Martínez Mayra Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a La Victoria km 0.6, C.P. 83304, Hermosillo, Sonora, México aut NATIONALLICENCE 700 1- López-Y-López Víctor Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizábal, C.P. 90700, Tlaxcala, México aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5439-5450 0175-7598 99:13<5439 2015 99 253