caa a22 4500 605506108 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6467-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6467-7 The distribution pattern of DNA and protoxin in Bacillus thuringiensis as revealed by laser confocal microscopy analysis [Elektronische Daten] [Quanfang Hu, Jingfang Wang, Zujiao Fu, Xiangtao Mo, Xuezhi Ding, Liqiu Xia, Youming Zhang, Yunjun Sun] It was reported that the parasporal crystal from Bacillus thuringiensis contained DNA fragments. To investigate the distribution of protoxin and DNA in B. thuringiensis cells at different growth stages, a cry1Ac-gfp fusion gene was constructed and expressed in an acrystalliferous B. thuringiensis strain, in which the localization of DNA and protoxin were indicated by DNA-specific dye and green fluorescent protein, respectively. When the recombinant cells were at the vegetative growth stage, the Cry1Ac-GFP fusion protein was not expressed and the DNA fluorescent signal was evenly distributed throughout the cell. At the initial stage of sporulation, the Cry1Ac-GFP fusion protein was expressed and accumulated as inclusion body, while two condensed DNA signals existed at each pole of the cell. With the extension of culture time, it seemed that the DNA fluorescence from the region of spore development gradually became faint or vanishing, while the DNA signal was still present in the other pole or the remaining area of the mother cell. Interestingly and unexpectedly, there was no DNA fluorescence signal in the region of the growing and mature inclusion body of Cry1Ac-GFP in B. thuringiensis cell, which might indicate that the DNA embodied in the inclusion body was not accessible to the DNA-specific dye. This was the first investigation devoted exclusively to the in vivo distribution of protoxin and DNA in B. thuringiensis at different growth stages. These data shed light on deeply understanding the process of sporulation and parasporal crystal formation as well as further exploring the interaction of DNA and protoxin in B. thuringiensis. Springer-Verlag Berlin Heidelberg, 2015 Bacillus thuringiensis nationallicence Protoxin nationallicence DNA nationallicence In vivo distribution nationallicence Laser confocal microscopy nationallicence Hu Quanfang College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Wang Jingfang College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Fu Zujiao College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Mo Xiangtao College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Ding Xuezhi College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Xia Liqiu College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Zhang Youming State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Sun Yunjun College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5605-5612 0175-7598 99:13<5605 2015 99 253 https://doi.org/10.1007/s00253-015-6467-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6467-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Quanfang College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jingfang College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Fu Zujiao College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Mo Xiangtao College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Ding Xuezhi College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Xia Liqiu College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Youming State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yunjun College of Life Science, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5605-5612 0175-7598 99:13<5605 2015 99 253