Genetic tools and techniques for Chlamydomonas reinhardtii
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| 005 | 20210128100625.0 | ||
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| 008 | 210128e20150701xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6698-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6698-7 | ||
| 100 | 1 | |a Mussgnug |D Jan |u Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615, Bielefeld, Germany |4 aut | |
| 245 | 1 | 0 | |a Genetic tools and techniques for Chlamydomonas reinhardtii |h [Elektronische Daten] |c [Jan Mussgnug] |
| 520 | 3 | |a The development of tools has always been a major driving force for the advancement of science. Optical microscopes were the first instruments that allowed discovery and descriptive studies of the subcellular features of microorganisms. Although optical and electron microscopes remained at the forefront of microbiological research tools since their inventions, the advent of molecular genetics brought about questions which had to be addressed with new "genetic tools”. The unicellular green microalgal genus Chlamydomonas, especially the most prominent species C. reinhardtii, has become a frequently used model organism for many diverse fields of research and molecular genetic analyses of C. reinhardtii, as well as the available genetic tools and techniques, have become increasingly sophisticated throughout the last decades. The aim of this review is to provide an overview of the molecular key features of C. reinhardtii and summarize the progress related to the development of tools and techniques for genetic engineering of this organism, from pioneering DNA transformation experiments to state-of-the-art techniques for targeted nuclear genome editing and high-throughput screening approaches. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Chlamydomonas reinhardtii |2 nationallicence | |
| 690 | 7 | |a Genetic engineering |2 nationallicence | |
| 690 | 7 | |a Gene silencing |2 nationallicence | |
| 690 | 7 | |a Molecular toolkit |2 nationallicence | |
| 690 | 7 | |a Promoters |2 nationallicence | |
| 690 | 7 | |a Selectable markers |2 nationallicence | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5407-5418 |x 0175-7598 |q 99:13<5407 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6698-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6698-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 100 |E 1- |a Mussgnug |D Jan |u Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615, Bielefeld, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/13(2015-07-01), 5407-5418 |x 0175-7598 |q 99:13<5407 |1 2015 |2 99 |o 253 | ||