caa a22 4500 605506116 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6698-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6698-7 Mussgnug Jan Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615, Bielefeld, Germany aut Genetic tools and techniques for Chlamydomonas reinhardtii [Elektronische Daten] [Jan Mussgnug] The development of tools has always been a major driving force for the advancement of science. Optical microscopes were the first instruments that allowed discovery and descriptive studies of the subcellular features of microorganisms. Although optical and electron microscopes remained at the forefront of microbiological research tools since their inventions, the advent of molecular genetics brought about questions which had to be addressed with new "genetic tools”. The unicellular green microalgal genus Chlamydomonas, especially the most prominent species C. reinhardtii, has become a frequently used model organism for many diverse fields of research and molecular genetic analyses of C. reinhardtii, as well as the available genetic tools and techniques, have become increasingly sophisticated throughout the last decades. The aim of this review is to provide an overview of the molecular key features of C. reinhardtii and summarize the progress related to the development of tools and techniques for genetic engineering of this organism, from pioneering DNA transformation experiments to state-of-the-art techniques for targeted nuclear genome editing and high-throughput screening approaches. Springer-Verlag Berlin Heidelberg, 2015 Chlamydomonas reinhardtii nationallicence Genetic engineering nationallicence Gene silencing nationallicence Molecular toolkit nationallicence Promoters nationallicence Selectable markers nationallicence Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5407-5418 0175-7598 99:13<5407 2015 99 253 https://doi.org/10.1007/s00253-015-6698-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6698-7 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Mussgnug Jan Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615, Bielefeld, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5407-5418 0175-7598 99:13<5407 2015 99 253