caa a22 4500 605506175 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6616-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6616-z Adaptive laboratory evolution of ethanologenic Zymomonas mobilis strain tolerant to furfural and acetic acid inhibitors [Elektronische Daten] [Zong-Xia Shui, Han Qin, Bo Wu, Zhi-yong Ruan, Lu-shang Wang, Fu-Rong Tan, Jing-Li Wang, Xiao-Yu Tang, Li-Chun Dai, Guo-Quan Hu, Ming-Xiong He] Furfural and acetic acid from lignocellulosic hydrolysates are the prevalent inhibitors to Zymomonas mobilis during cellulosic ethanol production. Developing a strain tolerant to furfural or acetic acid inhibitors is difficul by using rational engineering strategies due to poor understanding of their underlying molecular mechanisms. In this study, strategy of adaptive laboratory evolution (ALE) was used for development of a furfural and acetic acid-tolerant strain. After three round evolution, four evolved mutants (ZMA7-2, ZMA7-3, ZMF3-2, and ZMF3-3) that showed higher growth capacity were successfully obtained via ALE method. Based on the results of profiling of cell growth, glucose utilization, ethanol yield, and activity of key enzymes, two desired strains, ZMA7-2 and ZMF3-3, were achieved, which showed higher tolerance under 7g/l acetic acid and 3g/l furfural stress condition. Especially, it is the first report of Z. mobilis strain that could tolerate higher furfural. The best strain, Z. mobilis ZMF3-3, has showed 94.84% theoretical ethanol yield under 3-g/l furfural stress condition, and the theoretical ethanol yield of ZM4 is only 9.89%. Our study also demonstrated that ALE method might also be used as a powerful metabolic engineering tool for metabolic engineering in Z. mobilis. Furthermore, the two best strains could be used as novel host for further metabolic engineering in cellulosic ethanol or future biorefinery. Importantly, the two strains may also be used as novel-tolerant model organisms for the genetic mechanism on the "omics” level, which will provide some useful information for inverse metabolic engineering. Springer-Verlag Berlin Heidelberg, 2015 Adaptive laboratory evolution (ALE) nationallicence Lignocellulosic hydrolysates nationallicence Furfural nationallicence Acetic acid nationallicence Bioethanol nationallicence Zymomonas mobilis nationallicence Shui Zong-Xia Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Qin Han Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Wu Bo Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Ruan Zhi-yong Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Wang Lu-shang State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Tan Fu-Rong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Wang Jing-Li Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Tang Xiao-Yu Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Dai Li-Chun Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Hu Guo-Quan Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut He Ming-Xiong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5739-5748 0175-7598 99:13<5739 2015 99 253 https://doi.org/10.1007/s00253-015-6616-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6616-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shui Zong-Xia Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Qin Han Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Wu Bo Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Ruan Zhi-yong Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Lu-shang State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Tan Fu-Rong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jing-Li Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Tang Xiao-Yu Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Dai Li-Chun Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Hu Guo-Quan Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- He Ming-Xiong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5739-5748 0175-7598 99:13<5739 2015 99 253