caa a22 4500 605506183 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6685-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6685-z Effects of impurities in biodiesel-derived glycerol on growth and expression of heavy metal ion homeostasis genes and gene products in Pseudomonas putida LS46 [Elektronische Daten] [Jilagamazhi Fu, Parveen Sharma, Vic Spicer, Oleg Krokhin, Xiangli Zhang, Brian Fristensky, John Wilkins, Nazim Cicek, Richard Sparling, David. Levin] Biodiesel production-derived waste glycerol (WG) was previously investigated as potential carbon source for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas putida LS46. In this study, we evaluated the effect of impurities in the WG on P. putida LS46 physiology during exponential growth and corresponding changes in transcription and protein expression profiles compared with cells grown on pure, reagent grade glycerol. High concentration of metal ions, such as Na+, and numbers of heavy metals ion, such as copper, ion, zinc, were detected in biodiesel-derived WG. Omics analysis from the corresponding cultures suggested altered expression of genes involved in transport and metabolism of ammonia and heavy metal ions. Expression of three groups of heavy metal homeostasis genes was significantly changed (mostly upregulated) in WG cultures and included the following: copper-responded cluster 1 and 2 genes, primarily containing cusABC; two copies of copAB and heavy metal translocating P-type ATPase; Fur-regulated, TonB-dependent siderophore receptor; and several cobalt/zinc/cadmium transporters. Expression of these genes suggests regulation of intracellular concentrations of heavy metals during growth on biodiesel-derived glycerol. Finally, a number of genes involved in adapting to, or metabolizing free fatty acids and other nonheavy metal contaminants, such as Na+, were also upregulated in P. putida LS46 grown on biodiesel-derived glycerol. Springer-Verlag Berlin Heidelberg, 2015 Omics nationallicence mcl-PHA nationallicence Heavy metals nationallicence P. putida LS46 nationallicence Biodiesel-derived waste glycerol nationallicence Fu Jilagamazhi Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut Sharma Parveen Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut Spicer Vic Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada aut Krokhin Oleg Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada aut Zhang Xiangli Department of Plant Science, University of Manitoba, Winnipeg, MB, Canada aut Fristensky Brian Department of Plant Science, University of Manitoba, Winnipeg, MB, Canada aut Wilkins John Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada aut Cicek Nazim Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut Sparling Richard Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada aut Levin David Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5583-5592 0175-7598 99:13<5583 2015 99 253 https://doi.org/10.1007/s00253-015-6685-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6685-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fu Jilagamazhi Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Sharma Parveen Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Spicer Vic Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Krokhin Oleg Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Zhang Xiangli Department of Plant Science, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Fristensky Brian Department of Plant Science, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Wilkins John Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Cicek Nazim Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Sparling Richard Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 700 1- Levin David Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/13(2015-07-01), 5583-5592 0175-7598 99:13<5583 2015 99 253