Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6396-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6396-5 | ||
| 245 | 0 | 0 | |a Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785 |h [Elektronische Daten] |c [Masahiro Kamon, Jun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi, M. Kamon, J. Sumitani, S. Tani, T. Kawaguchi] |
| 520 | 3 | |a A maltotriose-forming amylase (G3Amy) from Kitasatospora sp. MK-1785 was successfully isolated from a soil sample by inhibiting typical extracellular α-amylases using a proteinaceous α-amylase inhibitor. G3Amy was purified from the MK-1785 culture supernatant and characterized. G3Amy produced maltotriose as the principal product from starch and was categorized as an exo-α-amylase. G3Amy could also transfer maltotriose to phenolic and alcoholic compounds. Therefore, G3Amy can be useful for not only maltotriose manufacture but also maltooligosaccharide-glycoside synthesis. Further, the G3Amy gene was cloned and expressed in Escherichia coli cells. Analysis of its deduced amino acid sequence revealed that G3Amy consisted of an N-terminal GH13 catalytic domain and two C-terminal repeat starch-binding domains belonging to CBM20. It is suggested that natural G3Amy was subjected to proteolysis at N-terminal region of the anterior CBM20 in the C-terminal region. As with natural G3Amy, recombinant G3Amy could produce and transfer maltotriose from starch. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Kitasatospora |2 nationallicence | |
| 690 | 7 | |a Exo-α-amylase |2 nationallicence | |
| 690 | 7 | |a Maltotriose |2 nationallicence | |
| 690 | 7 | |a Transglycosylation |2 nationallicence | |
| 690 | 7 | |a Cloning |2 nationallicence | |
| 700 | 1 | |a Kamon |D Masahiro |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Sumitani |D Jun-ichi |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Tani |D Shuji |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Kawaguchi |D Takashi |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Kamon |D M. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Sumitani |D J. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Tani |D S. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 700 | 1 | |a Kawaguchi |D T. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4743-4753 |x 0175-7598 |q 99:11<4743 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6396-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6396-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kamon |D Masahiro |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sumitani |D Jun-ichi |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tani |D Shuji |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawaguchi |D Takashi |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kamon |D M. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sumitani |D J. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tani |D S. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawaguchi |D T. |u Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4743-4753 |x 0175-7598 |q 99:11<4743 |1 2015 |2 99 |o 253 | ||