caa a22 4500 605506191 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6396-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6396-5 Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785 [Elektronische Daten] [Masahiro Kamon, Jun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi, M. Kamon, J. Sumitani, S. Tani, T. Kawaguchi] A maltotriose-forming amylase (G3Amy) from Kitasatospora sp. MK-1785 was successfully isolated from a soil sample by inhibiting typical extracellular α-amylases using a proteinaceous α-amylase inhibitor. G3Amy was purified from the MK-1785 culture supernatant and characterized. G3Amy produced maltotriose as the principal product from starch and was categorized as an exo-α-amylase. G3Amy could also transfer maltotriose to phenolic and alcoholic compounds. Therefore, G3Amy can be useful for not only maltotriose manufacture but also maltooligosaccharide-glycoside synthesis. Further, the G3Amy gene was cloned and expressed in Escherichia coli cells. Analysis of its deduced amino acid sequence revealed that G3Amy consisted of an N-terminal GH13 catalytic domain and two C-terminal repeat starch-binding domains belonging to CBM20. It is suggested that natural G3Amy was subjected to proteolysis at N-terminal region of the anterior CBM20 in the C-terminal region. As with natural G3Amy, recombinant G3Amy could produce and transfer maltotriose from starch. Springer-Verlag Berlin Heidelberg, 2015 Kitasatospora nationallicence Exo-α-amylase nationallicence Maltotriose nationallicence Transglycosylation nationallicence Cloning nationallicence Kamon Masahiro Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Sumitani Jun-ichi Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Tani Shuji Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Kawaguchi Takashi Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Kamon M. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Sumitani J. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Tani S. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Kawaguchi T. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4743-4753 0175-7598 99:11<4743 2015 99 253 https://doi.org/10.1007/s00253-015-6396-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6396-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamon Masahiro Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Sumitani Jun-ichi Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Tani Shuji Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Kawaguchi Takashi Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Kamon M. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Sumitani J. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Tani S. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Kawaguchi T. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4743-4753 0175-7598 99:11<4743 2015 99 253