caa a22 4500 605506205 CHVBK 20210128100625.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6513-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6513-5 Acetate is a superior substrate for microbial fuel cell initiation preceding bioethanol effluent utilization [Elektronische Daten] [Guotao Sun, Anders Thygesen, Anne Meyer] This study assessed cell voltage development, electricity recovery, and microbial community composition in response to initial substrate including acetate, xylose, acetate/xylose 1:1 mixture (ace/xyl), and bioethanol effluent (BE) during microbial fuel cell (MFC) operation at 1000 Ω external resistance. The BE mainly contained 20.5g/L xylose, 1.8g/L arabinose, and 2.5g/L propionic acid. The MFCs initially fed with acetate showed shorter initiation time (1day), higher average cell voltage (634 ± 9mV), and higher coulombic efficiency (31.5 ± 0.5%) than those initially fed with ace/xyl or xylose. However, BE-initiated MFCs only generated 162 ± 1mV. The acetate-initiated MFCs exhibited longer adaptation time (21h) and lower cell voltage (645 ± 10mV) when the substrate was switched to xylose, whereas substrate switching to BE produced the highest voltage (656mV), maximum power density (362 ± 27mW/m2), maximum current density (709 ± 27mA/m2), and coulombic efficiency (25 ± 0.5%) in the acetate-initiated MFCs. The microbial community in acetate-initiated MFCs was less diverse and contained more electrogenic bacteria (13.9 ± 0.4%) including Geobacter sulfurreducens and Desulfuromonas acetexigen than the MFCs initially fed with ace/xyl, xylose, and BE. After switching the substrate to xylose and subsequently to BE, the microbial community in the acetate-initiated MFCs became more diverse, while no significant changes were observed in ace/xyl-, xylose-, and BE-initiated MFCs. The results showed that initial substrate affected the power generation and the capability to adapt to the substrate alteration in MFCs. Acetate-initiated MFCs showed best performance in utilizing BE. Springer-Verlag Berlin Heidelberg, 2015 Microbial fuel cell nationallicence Bioethanol effluent nationallicence Denaturing gradient gel electrophoresis nationallicence Biofilm nationallicence Coulombic efficiency nationallicence Sun Guotao Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, DK-2800, Kgs. Lyngby, Denmark aut Thygesen Anders Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, DK-2800, Kgs. Lyngby, Denmark aut Meyer Anne Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, DK-2800, Kgs. Lyngby, Denmark aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4905-4915 0175-7598 99:11<4905 2015 99 253 https://doi.org/10.1007/s00253-015-6513-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6513-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Guotao Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, DK-2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Thygesen Anders Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, DK-2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Meyer Anne Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, DK-2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4905-4915 0175-7598 99:11<4905 2015 99 253