caa a22 4500 605506213 CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6415-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6415-6 Chilli leaf curl virus infection highlights the differential expression of genes involved in protein homeostasis and defense in resistant chilli plants [Elektronische Daten] [Nirbhay Kushwaha, Pranav Sahu, Manoj Prasad, Supriya Chakraborty] Geminiviruses have evolved with tremendous potential of recombination and possess the ability to manipulate several cellular processes of hosts. Chilli leaf curl virus (ChiLCV) is a monopartite Begomovirus (family Geminiviridae) which has emerged as a serious threat to chilli production worldwide. To date, development of resistant chilli varieties through conventional plant breeding techniques remains the major antiviral strategy. To explore the potential resistance factors in Capsicum annuum var. Punjab Lal, we performed a transcriptome analysis in ChiLCV-infected plants by exploiting the advantage of sensitivity and efficiency of suppression subtractive hybridization (SSH). Out of 480 clones screened, 231 unique expressed sequence tags (ESTs) involved in different cellular and physiological processes were identified. An interactome network of ChiLCV responsive differentially expressed genes revealed an array of proteins involved in key cellular processes including transcription, replication, photosynthesis, and defense. A comparative study of gene expression between resistant and susceptible chilli plants revealed upregulation of several defense-related genes such as nucleotide-binding site leucine-rich repeat (NBS-LRR) domain containing protein, lipid transfer protein, thionin, polyphenol oxidase, and other proteins like ATP/ADP transporter in the ChiLCV-resistant variety. Taken together, the present study provides novel insights into the transcriptomics of ChiLCV-resistant chilli plants. Springer-Verlag Berlin Heidelberg, 2015 Geminivirus nationallicence Transcriptome analysis nationallicence Host gene expression nationallicence Resistance nationallicence Chilli nationallicence Suppression subtractive hybridization nationallicence Kushwaha Nirbhay Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut Sahu Pranav Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut Prasad Manoj National Institute of Plant Genome Research, 110067, New Delhi, India aut Chakraborty Supriya Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4757-4770 0175-7598 99:11<4757 2015 99 253 https://doi.org/10.1007/s00253-015-6415-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6415-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kushwaha Nirbhay Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Sahu Pranav Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Prasad Manoj National Institute of Plant Genome Research, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Chakraborty Supriya Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4757-4770 0175-7598 99:11<4757 2015 99 253