caa a22 4500 60550623X CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6349-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6349-4 Effectiveness and toxicity of a novel isolated actinomycete strain Streptomyces sp. JS01 on a harmful alga Phaeocystis globosa [Elektronische Daten] [Huajun Zhang, Su Zhang, Yun Peng, Yi Li, Guanjing Cai, Zhangran Chen, Wei Zheng, Yun Tian, Hong Xu, Tianling Zheng] An aquatic actinomycete capable of eliminating the brown tide causing marine alga Phaeocystis globosa was isolated from the surface sea water and the isolate named JS01 was characterized as Streptomyces on the basis of its 16S rRNA gene sequence. The supernatant of JS01 could lyse algal cells, implying that JS01 produced a latent alga-lytic compound. Considering this algicidal activity and the response of the algal cells, Chlorophyll a fluorescence decreased significantly in P. globosa in response to the JS01 supernatant when analyzed with flow cytometry. The algal cells experienced cell shrinkage and plasmolysis before disintegration after 72h of treatment. The released algicide(s) were heat-tolerant, except above 121°C, and fluctuation in pH variations; even so, algicidal activity was also over 60%. The maximum toxicity of JS01 was on the seventh day of culture, and the relative luminosity was 0.49 at that time when detected by luminous bacteria Vibrio fischeri. These results indicated that the Streptomyces sp. JS01 could function as a potential controller of Phaeocystis globosa blooms. Springer-Verlag Berlin Heidelberg, 2015 Phaeocystis globosa nationallicence Algicidal activity nationallicence Streptomyces nationallicence Harmful alga blooms nationallicence Toxicity nationallicence Zhang Huajun State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Zhang Su State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Peng Yun State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Li Yi State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Cai Guanjing State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Chen Zhangran State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Zheng Wei State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Tian Yun State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Xu Hong State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Zheng Tianling State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4807-4814 0175-7598 99:11<4807 2015 99 253 https://doi.org/10.1007/s00253-014-6349-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6349-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Huajun State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Zhang Su State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Peng Yun State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Li Yi State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Cai Guanjing State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Chen Zhangran State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Zheng Wei State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Tian Yun State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Xu Hong State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 700 1- Zheng Tianling State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, 361102, Xiamen, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4807-4814 0175-7598 99:11<4807 2015 99 253