caa a22 4500 605506248 CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6458-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6458-8 Overexpression of ribosome elongation factor G and recycling factor increases l -isoleucine production in Corynebacterium glutamicum [Elektronische Daten] [Jianxun Zhao, Xiaoqing Hu, Ye Li, Xiaoyuan Wang] Ribosome elongation factor G encoded by fusA promotes the translocation step of protein synthesis in bacteria; ribosome recycling factor encoded by frr, together with the elongation factor G, dissociates ribosomes from messenger RNA after the termination of translation. Both factors play important roles during protein synthesis in bacteria. In this study, we found that overexpression of fusA and/or frr led to the increase of l-isoleucine production in Corynebacterium glutamicum IWJ001, an l-isoleucine production strain generated by random mutagenesis. Reverse transcription polymerase chain reaction analysis showed that transcriptional levels of genes lysC, hom, thrB, ilvA, ilvBN, and ilvE encoding the key enzymes in the biosynthetic pathway of l-isoleucine increased in C. glutamicum IWJ001 when fusA and/or frr were overexpressed. Co-overexpression of fusA and frr, together with genes ilvA, ilvB, ilvN, and ppnk in C. glutamicum IWJ001, led to 76.5% increase of l-isoleucine production in flask cultivation and produced 28.5g/L l-isoleucine in 72-h fed-batch fermentation. The results demonstrate that overexpressing ribosome elongation factor G and ribosome recycling factor is an efficient approach to enhance l-isoleucine production in C. glutamicum. Springer-Verlag Berlin Heidelberg, 2015 Corynebacterium glutamicum nationallicence l -Isoleucine production nationallicence fusA nationallicence frr nationallicence Elongation factor G nationallicence Ribosome recycling factor nationallicence Zhao Jianxun State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut Hu Xiaoqing State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut Li Ye State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut Wang Xiaoyuan State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4795-4805 0175-7598 99:11<4795 2015 99 253 https://doi.org/10.1007/s00253-015-6458-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6458-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Jianxun State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Hu Xiaoqing State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Li Ye State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 700 1- Wang Xiaoyuan State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4795-4805 0175-7598 99:11<4795 2015 99 253