caa a22 4500 605506256 CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6642-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6642-x Fernández-Lucas Jesús Applied Biotechnology Group, Department of Pharmacy and Biotechnology, Faculty of Biomedical Sciences, European University of Madrid, Urbanización El Bosque, Calle Tajo, s/n, 28670, Villaviciosa de Odón, Madrid, Spain aut Multienzymatic synthesis of nucleic acid derivatives: a general perspective [Elektronische Daten] [Jesús Fernández-Lucas] Living cells are most perfect synthetic factory. The surprising synthetic efficiency of biological systems is allowed by the combination of multiple processes catalyzed by enzymes working sequentially. In this sense, biocatalysis tries to reproduce nature's synthetic strategies to perform the synthesis of different organic compounds using natural catalysts such as cells or enzymes. Nowadays, the use of multienzymatic systems in biocatalysis is becoming a habitual strategy for the synthesis of organic compounds that leads to the realization of complex synthetic schemes. By combining several steps in one pot, a significant step economy can be realized and the potential for environmentally benign synthesis is improved. Using this sustainable synthetic system, several work-up steps can be avoided and pure products are ideally isolated after a series of reactions in one single vessel after just one straightforward purification step. In recent years, enzymatic methodology for the preparation of nucleic acid derivatives (NADs) has become a standard technique for the synthesis of a wide variety of natural NADs. Enzymatic methods have been shown to be an efficient alternative for the synthesis of nucleoside and nucleotide analogs to the traditional multistep chemical methods, since chemical glycosylation reactions include several protection-deprotection steps and the use of chemical reagents and organic solvents that are expensive and environmentally harmful. In this minireview, we want to illustrate what we consider the most current relevant examples of in vivo and in vitro multienzymatic systems used for the synthesis of nucleic acid derivatives showing advantages and disadvantages of each methodology. Finally, a detailed perspective about the impact of -omics in multienzymatic systems has been described. Springer-Verlag Berlin Heidelberg, 2015 NADs nationallicence Multienzymatic systems nationallicence Cells nationallicence Biocatalysis nationallicence Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4615-4627 0175-7598 99:11<4615 2015 99 253 https://doi.org/10.1007/s00253-015-6642-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6642-x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Fernández-Lucas Jesús Applied Biotechnology Group, Department of Pharmacy and Biotechnology, Faculty of Biomedical Sciences, European University of Madrid, Urbanización El Bosque, Calle Tajo, s/n, 28670, Villaviciosa de Odón, Madrid, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4615-4627 0175-7598 99:11<4615 2015 99 253