Metabolic engineering of Clostridium tyrobutyricum for n -butanol production: effects of CoA transferase
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6566-5 |2 doi |
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| 245 | 0 | 0 | |a Metabolic engineering of Clostridium tyrobutyricum for n -butanol production: effects of CoA transferase |h [Elektronische Daten] |c [Le Yu, Jingbo Zhao, Mengmeng Xu, Jie Dong, Saju Varghese, Mingrui Yu, I-Ching Tang, Shang-Tian Yang] |
| 520 | 3 | |a The overexpression of CoA transferase (ctfAB), which catalyzes the reaction: acetate/butyrate + acetoacetyl-CoA → acetyl/butyryl-CoA + acetoacetate, was studied for its effects on acid reassimilation and butanol biosynthesis in Clostridium tyrobutyricum (Δack, adhE2). The plasmid pMTL007 was used to co-express adhE2 and ctfAB from Clostridium acetobutylicum ATCC 824. In addition, the sol operon containing ctfAB, adc (acetoacetate decarboxylase), and ald (aldehyde dehydrogenase) was also cloned from Clostridium beijerinckii NCIMB 8052 and expressed in C. tyrobutyricum (Δack, adhE2). Mutants expressing these genes were evaluated for their ability to produce butanol from glucose in batch fermentations at pH5.0 and 6.0. Compared to C. tyrobutyricum (Δack, adhE2) without expressing ctfAB, all mutants with ctfAB overexpression produced more butanol, with butanol yield increased to 0.22 − 0.26g/g (vs. 0.10 − 0.13g/g) and productivity to 0.35g/lh (vs. 0.13g/lh) because of the reduced acetate and butyrate production. The expression of ctfAB also resulted in acetone production from acetoacetate through a non-enzymatic decarboxylation. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Aldehyde/alcohol dehydrogenase |2 nationallicence | |
| 690 | 7 | |a Butanol |2 nationallicence | |
| 690 | 7 | |a Clostridium tyrobutyricum |2 nationallicence | |
| 690 | 7 | |a CoA transferase |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 700 | 1 | |a Yu |D Le |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Zhao |D Jingbo |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Xu |D Mengmeng |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Dong |D Jie |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Varghese |D Saju |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Yu |D Mingrui |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Tang |D I-Ching |u Bioprocessing Innovative Company, 4734 Bridle Path Ct., 43017, Dublin, OH, USA |4 aut | |
| 700 | 1 | |a Yang |D Shang-Tian |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4917-4930 |x 0175-7598 |q 99:11<4917 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6566-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
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| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6566-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Le |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhao |D Jingbo |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Mengmeng |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dong |D Jie |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Varghese |D Saju |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Mingrui |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tang |D I-Ching |u Bioprocessing Innovative Company, 4734 Bridle Path Ct., 43017, Dublin, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Shang-Tian |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4917-4930 |x 0175-7598 |q 99:11<4917 |1 2015 |2 99 |o 253 | ||