caa a22 4500 605506264 CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6566-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6566-5 Metabolic engineering of Clostridium tyrobutyricum for n -butanol production: effects of CoA transferase [Elektronische Daten] [Le Yu, Jingbo Zhao, Mengmeng Xu, Jie Dong, Saju Varghese, Mingrui Yu, I-Ching Tang, Shang-Tian Yang] The overexpression of CoA transferase (ctfAB), which catalyzes the reaction: acetate/butyrate + acetoacetyl-CoA → acetyl/butyryl-CoA + acetoacetate, was studied for its effects on acid reassimilation and butanol biosynthesis in Clostridium tyrobutyricum (Δack, adhE2). The plasmid pMTL007 was used to co-express adhE2 and ctfAB from Clostridium acetobutylicum ATCC 824. In addition, the sol operon containing ctfAB, adc (acetoacetate decarboxylase), and ald (aldehyde dehydrogenase) was also cloned from Clostridium beijerinckii NCIMB 8052 and expressed in C. tyrobutyricum (Δack, adhE2). Mutants expressing these genes were evaluated for their ability to produce butanol from glucose in batch fermentations at pH5.0 and 6.0. Compared to C. tyrobutyricum (Δack, adhE2) without expressing ctfAB, all mutants with ctfAB overexpression produced more butanol, with butanol yield increased to 0.22 − 0.26g/g (vs. 0.10 − 0.13g/g) and productivity to 0.35g/lh (vs. 0.13g/lh) because of the reduced acetate and butyrate production. The expression of ctfAB also resulted in acetone production from acetoacetate through a non-enzymatic decarboxylation. Springer-Verlag Berlin Heidelberg, 2015 Aldehyde/alcohol dehydrogenase nationallicence Butanol nationallicence Clostridium tyrobutyricum nationallicence CoA transferase nationallicence Metabolic engineering nationallicence Yu Le William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Zhao Jingbo William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Xu Mengmeng William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Dong Jie William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Varghese Saju William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Yu Mingrui William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Tang I-Ching Bioprocessing Innovative Company, 4734 Bridle Path Ct., 43017, Dublin, OH, USA aut Yang Shang-Tian William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4917-4930 0175-7598 99:11<4917 2015 99 253 https://doi.org/10.1007/s00253-015-6566-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6566-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Le William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Zhao Jingbo William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Xu Mengmeng William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Dong Jie William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Varghese Saju William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Yu Mingrui William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Tang I-Ching Bioprocessing Innovative Company, 4734 Bridle Path Ct., 43017, Dublin, OH, USA aut NATIONALLICENCE 700 1- Yang Shang-Tian William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., 43210, Columbus, OH, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4917-4930 0175-7598 99:11<4917 2015 99 253