caa a22 4500 605506310 CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6348-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6348-5 Spatio-temporal variability of airborne bacterial communities and their correlation with particulate matter chemical composition across two urban areas [Elektronische Daten] [I. Gandolfi, V. Bertolini, G. Bestetti, R. Ambrosini, E. Innocente, G. Rampazzo, M. Papacchini, A. Franzetti] The study of spatio-temporal variability of airborne bacterial communities has recently gained importance due to the evidence that airborne bacteria are involved in atmospheric processes and can affect human health. In this work, we described the structure of airborne microbial communities in two urban areas (Milan and Venice, Northern Italy) through the sequencing, by the Illumina platform, of libraries containing the V5-V6 hypervariable regions of the 16S rRNA gene and estimated the abundance of airborne bacteria with quantitative PCR (qPCR). Airborne microbial communities were dominated by few taxa, particularly Burkholderiales and Actinomycetales, more abundant in colder seasons, and Chloroplasts, more abundant in warmer seasons. By partitioning the variation in bacterial community structure, we could assess that environmental and meteorological conditions, including variability between cities and seasons, were the major determinants of the observed variation in bacterial community structure, while chemical composition of atmospheric particulate matter (PM) had a minor contribution. Particularly, Ba, SO4 2− and Mg2+ concentrations were significantly correlated with microbial community structure, but it was not possible to assess whether they simply co-varied with seasonal shifts of bacterial inputs to the atmosphere, or their variation favoured specific taxa. Both local sources of bacteria and atmospheric dispersal were involved in the assembling of airborne microbial communities, as suggested, to the one side by the large abundance of bacteria typical of lagoon environments (Rhodobacterales) observed in spring air samples from Venice and to the other by the significant effect of wind speed in shaping airborne bacterial communities at all sites. Springer-Verlag Berlin Heidelberg, 2015 Air pollution nationallicence Bioaerosol nationallicence Particulate matter nationallicence NGS nationallicence Milan nationallicence Venice nationallicence Gandolfi I. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut Bertolini V. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut Bestetti G. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut Ambrosini R. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut Innocente E. Department of Environmental Sciences, Informatics and Statistics, University of Ca' Foscari, Dorsoduro 2137, 30123, Venice, Italy aut Rampazzo G. Department of Environmental Sciences, Informatics and Statistics, University of Ca' Foscari, Dorsoduro 2137, 30123, Venice, Italy aut Papacchini M. INAIL Settore Ricerca, Certificazione e Verifica—DIPIA, Roma, Italy aut Franzetti A. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4867-4877 0175-7598 99:11<4867 2015 99 253 https://doi.org/10.1007/s00253-014-6348-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6348-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gandolfi I. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Bertolini V. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Bestetti G. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Ambrosini R. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Innocente E. Department of Environmental Sciences, Informatics and Statistics, University of Ca' Foscari, Dorsoduro 2137, 30123, Venice, Italy aut NATIONALLICENCE 700 1- Rampazzo G. Department of Environmental Sciences, Informatics and Statistics, University of Ca' Foscari, Dorsoduro 2137, 30123, Venice, Italy aut NATIONALLICENCE 700 1- Papacchini M. INAIL Settore Ricerca, Certificazione e Verifica—DIPIA, Roma, Italy aut NATIONALLICENCE 700 1- Franzetti A. Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4867-4877 0175-7598 99:11<4867 2015 99 253