Design of N -acyl homoserine lactonase with high substrate specificity by a rational approach
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| 008 | 210128e20150601xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6304-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6304-4 | ||
| 245 | 0 | 0 | |a Design of N -acyl homoserine lactonase with high substrate specificity by a rational approach |h [Elektronische Daten] |c [Hyun-Ho Kyeong, Jin-Hyun Kim, Hak-Sung Kim] |
| 520 | 3 | |a N-Acyl homoserine lactone (AHL) is a major quorum-sensing signaling molecule in many bacterial species. Quorum-quenching (QQ) enzymes, which degrade such signaling molecules, have attracted much attention as an approach to controlling and preventing bacterial virulence and pathogenesis. However, naturally occurring QQ enzymes show a broad substrate spectrum, raising the concern of unintentionally attenuating beneficial effects by symbiotic bacteria. Here we report the rational design of acyl homoserine lactonase with high substrate specificity. Through docking analysis, we identified three key residues which play a key role in the substrate preference of the enzyme. The key residues were changed in a way that increases hydrophobic contact with a substrate having a short acyl chain (C4-AHL) while generating steric clashes with that containing a long acyl chain (C12-AHL). The resulting mutants exhibited a significantly shifted preference toward a substrate with a short acyl chain. Molecular dynamics simulations suggested that the mutations affect the behavior of a flexible loop, allowing tighter binding of a substrate with a short acyl chain. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Quorum quenching |2 nationallicence | |
| 690 | 7 | |a Acyl homoserine lactonase |2 nationallicence | |
| 690 | 7 | |a Substrate preference |2 nationallicence | |
| 690 | 7 | |a Rational design |2 nationallicence | |
| 700 | 1 | |a Kyeong |D Hyun-Ho |u Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea |4 aut | |
| 700 | 1 | |a Kim |D Jin-Hyun |u LG Household & Health Care Ltd., R&D Institute, 305-343, Daejeon, Korea |4 aut | |
| 700 | 1 | |a Kim |D Hak-Sung |u Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4735-4742 |x 0175-7598 |q 99:11<4735 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6304-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6304-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kyeong |D Hyun-Ho |u Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Jin-Hyun |u LG Household & Health Care Ltd., R&D Institute, 305-343, Daejeon, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Hak-Sung |u Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4735-4742 |x 0175-7598 |q 99:11<4735 |1 2015 |2 99 |o 253 | ||