The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6445-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6445-0 | ||
| 245 | 0 | 4 | |a The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering |h [Elektronische Daten] |c [Adwoa Baah-Dwomoh, Andrea Rolong, Paul Gatenholm, Rafael Davalos] |
| 520 | 3 | |a This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of G. xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 to 12.5kV/cm, producing a local field of 3 kV/cm, was sufficient to kill most ofthe bacteria and create a localized pore. However,an applied electric field of 17.5kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Bacterial cellulose |2 nationallicence | |
| 690 | 7 | |a Irreversible electroporation |2 nationallicence | |
| 690 | 7 | |a Scaffolds for tissue engineering |2 nationallicence | |
| 690 | 7 | |a Biofabrication |2 nationallicence | |
| 700 | 1 | |a Baah-Dwomoh |D Adwoa |u Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA |4 aut | |
| 700 | 1 | |a Rolong |D Andrea |u School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA |4 aut | |
| 700 | 1 | |a Gatenholm |D Paul |u School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA |4 aut | |
| 700 | 1 | |a Davalos |D Rafael |u Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4785-4794 |x 0175-7598 |q 99:11<4785 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6445-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6445-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Baah-Dwomoh |D Adwoa |u Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rolong |D Andrea |u School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gatenholm |D Paul |u School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Davalos |D Rafael |u Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4785-4794 |x 0175-7598 |q 99:11<4785 |1 2015 |2 99 |o 253 | ||