caa a22 4500 60550637X CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6445-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6445-0 The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering [Elektronische Daten] [Adwoa Baah-Dwomoh, Andrea Rolong, Paul Gatenholm, Rafael Davalos] This work investigates the feasibility of the use of irreversible electroporation (IRE) in the biofabrication of 3D cellulose nanofibril networks via the bacterial strain Gluconacetobacter xylinus. IRE uses electrical pulses to increase membrane permeability by altering the transmembrane potential; past a threshold, damage to the cell becomes too great and leads to cell death. We hypothesized that using IRE to kill the bacteria at specific locations and particular times, we could introduce conduits in the overall scaffold by preventing cellulose biosynthesis locally. Through mathematical modeling and experimental techniques, electrical effects were investigated and the parameters for IRE of G. xylinus were determined. We found that for a specific set of parameters, an applied electric field of 8 to 12.5kV/cm, producing a local field of 3 kV/cm, was sufficient to kill most ofthe bacteria and create a localized pore. However,an applied electric field of 17.5kV/cm was required to kill all. Results suggest that IRE may be an effective tool to create scaffolds with appropriate porosity for orthopedic applications. Ideally, these engineered scaffolds could be used to successfully treat osteochondral defects. Springer-Verlag Berlin Heidelberg, 2015 Bacterial cellulose nationallicence Irreversible electroporation nationallicence Scaffolds for tissue engineering nationallicence Biofabrication nationallicence Baah-Dwomoh Adwoa Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA aut Rolong Andrea School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA aut Gatenholm Paul School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA aut Davalos Rafael Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4785-4794 0175-7598 99:11<4785 2015 99 253 https://doi.org/10.1007/s00253-015-6445-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6445-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baah-Dwomoh Adwoa Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Rolong Andrea School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Gatenholm Paul School of Biomedical Engineering and Sciences, Virginia Tech—Wake Forest University, 329 Kelly Hall, 325 Stanger Street (MC 0298), 24061, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Davalos Rafael Materials Science and Engineering, Virginia Tech, Blacksburg, VA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4785-4794 0175-7598 99:11<4785 2015 99 253