Production of pinoresinol diglucoside, pinoresinol monoglucoside, and pinoresinol by Phomopsis sp. XP-8 using mung bean and its major components
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6491-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6491-7 | ||
| 245 | 0 | 0 | |a Production of pinoresinol diglucoside, pinoresinol monoglucoside, and pinoresinol by Phomopsis sp. XP-8 using mung bean and its major components |h [Elektronische Daten] |c [Yan Zhang, Junling Shi, Zhenhong Gao, Ruiming Yangwu, Huanshi Jiang, Jinxin Che, Yanlin Liu] |
| 520 | 3 | |a Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and pinoresinol (Pin) were also found in the culture medium. In this experiment, starch, protein, and polysaccharides were isolated from mung beans and separately used as the sole substrate in order to explore the mechanism of fermentation and identify the major substrates that attributed to the biotransformation of PDG, PMG, and Pin. The production of PDG, PMG, and Pin was monitored using high-performance liquid chromatography (HPLC) and confirmed using HPLC-MS. Activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL) were analyzed and tracked during the cultivation. The reaction system contained the compounds isolated from mung bean in the designed amount. Accumulation of phenylalanine, cinnamic acid, p-coumaric acid, PDG, PMG, and Pin and the activities of PAL, C4H, and 4CL were measured during the bioconversion. PMG was found only when mung bean polysaccharide was analyzed, while production of PDG and Pin were found when both polysaccharide and starch were analyzed. After examining the monosaccharide composition of the mung bean polysaccharide and the effect of the different monosaccharides had on the production of PMG, PDG, and Pin, galactose in mung bean polysaccharide proved to be the major factor that stimulates the production of PMG. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Phomopsis sp. XP-8 |2 nationallicence | |
| 690 | 7 | |a Bioconversion |2 nationallicence | |
| 690 | 7 | |a Pinoresinol diglucoside |2 nationallicence | |
| 690 | 7 | |a Pinoresinol monoglucoside |2 nationallicence | |
| 690 | 7 | |a Pinoresinol |2 nationallicence | |
| 700 | 1 | |a Zhang |D Yan |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | |
| 700 | 1 | |a Shi |D Junling |u Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, 710072, Xi'an, Shaanxi Province, China |4 aut | |
| 700 | 1 | |a Gao |D Zhenhong |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | |
| 700 | 1 | |a Yangwu |D Ruiming |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | |
| 700 | 1 | |a Jiang |D Huanshi |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | |
| 700 | 1 | |a Che |D Jinxin |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | |
| 700 | 1 | |a Liu |D Yanlin |u College of Enology, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4629-4643 |x 0175-7598 |q 99:11<4629 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6491-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6491-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Yan |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shi |D Junling |u Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, 710072, Xi'an, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Zhenhong |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yangwu |D Ruiming |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jiang |D Huanshi |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Che |D Jinxin |u College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Yanlin |u College of Enology, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/11(2015-06-01), 4629-4643 |x 0175-7598 |q 99:11<4629 |1 2015 |2 99 |o 253 | ||