caa a22 4500 605506388 CHVBK 20210128100626.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6491-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6491-7 Production of pinoresinol diglucoside, pinoresinol monoglucoside, and pinoresinol by Phomopsis sp. XP-8 using mung bean and its major components [Elektronische Daten] [Yan Zhang, Junling Shi, Zhenhong Gao, Ruiming Yangwu, Huanshi Jiang, Jinxin Che, Yanlin Liu] Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and pinoresinol (Pin) were also found in the culture medium. In this experiment, starch, protein, and polysaccharides were isolated from mung beans and separately used as the sole substrate in order to explore the mechanism of fermentation and identify the major substrates that attributed to the biotransformation of PDG, PMG, and Pin. The production of PDG, PMG, and Pin was monitored using high-performance liquid chromatography (HPLC) and confirmed using HPLC-MS. Activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL) were analyzed and tracked during the cultivation. The reaction system contained the compounds isolated from mung bean in the designed amount. Accumulation of phenylalanine, cinnamic acid, p-coumaric acid, PDG, PMG, and Pin and the activities of PAL, C4H, and 4CL were measured during the bioconversion. PMG was found only when mung bean polysaccharide was analyzed, while production of PDG and Pin were found when both polysaccharide and starch were analyzed. After examining the monosaccharide composition of the mung bean polysaccharide and the effect of the different monosaccharides had on the production of PMG, PDG, and Pin, galactose in mung bean polysaccharide proved to be the major factor that stimulates the production of PMG. Springer-Verlag Berlin Heidelberg, 2015 Phomopsis sp. XP-8 nationallicence Bioconversion nationallicence Pinoresinol diglucoside nationallicence Pinoresinol monoglucoside nationallicence Pinoresinol nationallicence Zhang Yan College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut Shi Junling Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, 710072, Xi'an, Shaanxi Province, China aut Gao Zhenhong College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut Yangwu Ruiming College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut Jiang Huanshi College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut Che Jinxin College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut Liu Yanlin College of Enology, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4629-4643 0175-7598 99:11<4629 2015 99 253 https://doi.org/10.1007/s00253-015-6491-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6491-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yan College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut NATIONALLICENCE 700 1- Shi Junling Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, 710072, Xi'an, Shaanxi Province, China aut NATIONALLICENCE 700 1- Gao Zhenhong College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut NATIONALLICENCE 700 1- Yangwu Ruiming College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut NATIONALLICENCE 700 1- Jiang Huanshi College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut NATIONALLICENCE 700 1- Che Jinxin College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut NATIONALLICENCE 700 1- Liu Yanlin College of Enology, Northwest A&F University, 28 Xinong Road, 712100, Yangling, Shaanxi Province, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/11(2015-06-01), 4629-4643 0175-7598 99:11<4629 2015 99 253